| 摘要 |
[Objectives] To investigate the optimal extraction conditions for anthocyanins from defatted Lycium ruthenicum Murray using ultrasonic-assisted solvent extraction. [Methods] Anthocyanins were extracted from wild L. ruthenicum in Qinghai Province using ultrasonic-assisted ethanol extraction. Through single-factor and orthogonal experiments, the optimal extraction conditions were determined as follows: temperature 50 ℃, solid-liquid ratio 1：15 (g/mL), ethanol concentration 60% (v/v), and ultrasonic extraction time 25 min. Under these conditions, the anthocyanin content of L. ruthenicum was quantified by UV-Vis spectrophotometry at 280 nm. [Results] The extraction yield of anthocyanins from wild Qinghai L. ruthenicum was 17.0 mg/g, which is superior to the yield of 10.0 mg/g obtained by water solvent extraction, representing a 0.7% increase in extraction rate. The anthocyanin content in L. ruthenicum from different regions was determined, revealing that samples from the Chaidamu area in Qinghai had the highest content (17.3 mg/g), while samples from the Gansu area had the lowest (12.0 mg/g). [Conclusions] Ultrasonic-assisted ethanol extraction technology offers advantages including rapid operation, low energy consumption, high extraction yield, simple detection, and safety. |
