Application and Prospects of CRISPR/Cas9 Genome Editing Technology in the Genetic Improvement of Fruit Trees
刊名 Asian Agricultural Research
作者 Xiaoqi CHEN, Shaoping WU
作者单位 State Key Laboratory for Conservation and Utilization of Bio-Resources, School of Life Sciences, Yunnan University; School of Life Sciences
DOI 10.19601/j.cnki.issn1943-9903.2025.06.009
年份 2025
刊期 6
页码 40-46
关键词 CRISPR/Cas9, Genome editing, Fruit trees, Genetic improvement, Germplasm enhancement
摘要 CRISPR/Cas9 technology, a revolutionary gene-editing tool, has rapidly garnered attention in plant science owing to its simplicity, high editing efficiency, and cost-effectiveness. Besides, it offers unprecedented precision and efficiency in the genetic improvement of fruit trees. To date, this technology has been widely utilized to enhance fruit quality, improve stress resistance, and mediate growth and development. These applications demonstrate its immense potential in fruit tree breeding. Looking ahead, advancements in editing efficiency, expanded application scopes, comprehensive safety assessments, and improved regulatory frameworks are expected to further broaden the role of CRISPR/Cas9 in fruit tree breeding, thereby driving the fruit tree industry toward higher yield, superior quality, enhanced stress resilience, higher efficiency, and contributing to global food security and sustainable agricultural development. This article outlines the fundamental principles of CRISPR/Cas9 gene editing technology, its applications in plants (including fruit trees), and its pivotal role in genetic improvement and germplasm innovation.