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 摘要： [Objectives] To analyze the chemical components of Meconopsis integrifolia (Maxim.) Franch total flavonoids (MITF). [Methods] The chemical components of MITF were identified by UPLC-Q-Exactive Orbitrap MS. The column was performed using ACQUITY UPLC HSS T3 (100 mm×2.1 mm, 1.8 μm) with 0.1% formic acid water (A)-acetonitrile (B) with gradient elution at 30 ℃, injection volume of 2 μL, and flow rate of 0.3 mL/min. Electrospray ion source adopted positive and negative ion detection mode with scanning range m/z 100-1 000. [Results] A total of 93 compounds were identified from MITF, including 57 flavonoids, 25 phenolic acids, 9 alkaloids and 2 others. Among them, dimethoxytaxifolin was identified as a possible new compound by SciFinder search, and 67 compounds were first identified in M. integrifolia. [Conclusions] This study provides a scientific foundation for clarifying the material basis of the efficacy of M. integrifolia and improving the quality standards.

 摘要： [Objectives] To establish a multi-indicator quality control method for the retention of Longqing Capsule based on the principle of prescription of Chinese medicine. [Methods] High performance liquid chromatography (HPLC) with ShimNex CS C₁₈ as the column; column temperature: 35 ℃; wavelength: 270 nm; methanol-0.1% phosphoric acid solution as the mobile phase with gradient elution. [Results] The 12 components of the retention of Longqing Capsule showed good linearity within the investigated range (r≥0.999 5), with the average spiked recoveries of 97.83%-100.52% and the RSD of 0.9%-2.1%. [Conclusions] The method is exclusive, sensitive, reproducible, simple and easy to use, and can provide a reference for the construction of the quality standard and control system of Longqing Capsule based on the theory of traditional Chinese medicine.

 摘要： [Objectives] To analyze the main chemical components in Cocculus laurifolius DC. by ultra-high performance liquid chromatography-quaternary rod/electrostatic field orbital hydrazine high resolution mass spectrometry. [Methods] Using Welch AQ-C₁₈ chromatographic column (150 mm×2.1 mm, 1.8 μm), gradient elution was performed with 0.1% formic acid aqueous solution (A)-methanol (B) as the mobile phase, and electrospray ESI ionization source and simultaneous mass spectrometry scanning mode of positive and negative ions were used. [Results] 26 kinds of chemical component were identified or inferred, including 3 organic acids, 5 flavonoids, 4 alkaloids, 1 coumarin and 13 others. [Conclusions] The UPLC-Q-Exactive HRMS technique is simple, which lays a foundation for the drug-efficacy material basis and medicinal quality evaluation of C. laurifolius DC.

 摘要： [Objectives] To identify the authenticity of Longgu from the microscopic, infrared spectrum and chemical composition, and provide references for the quality control and evaluation methods of Longgu. [Methods] According to the mineral characteristics of Longgu, the identification research was carried out by microscope observation, near-infrared spectroscopy and X-ray fluorescence spectrometer. By comparing the single polarizing and orthogonal polarizing characteristics of genuine and fake Longgu, a qualitative identification model of genuine Longgu was established based on the near-infrared spectrum of genuine Longgu, and the detection results of elements in Longgu were analyzed. [Results] The genuine Longgu had apatite optical properties, and was quite different from the fake Longgu of animal bones. Compared with modern animal bones, genuine Longgus had relatively less P and Ca, but they were enriched in elements Sr and F. The correlation coefficient model with good predictive ability can be established by using the near-infrared characteristic spectrum. [Conclusions] Polarizing microscope, near-infrared spectroscopy and X-ray fluorescence spectroscopy can improve the identification results of Longgu.

 摘要： The latest progress in the process optimization and stability improvement of third-generation cephalosporins in recent years was reviewed. The introduction of green chemistry, enzyme catalysis, nanotechnology, lyophilization, and nitrogen-filled packaging technologies can only improve production efficiency and reduce the generation of by-products, but also significantly extend the shelf life of drugs. In the future, process automation and intelligent technology will further optimize the large-scale production process, and the combination of nanotechnology and precision drug delivery will promote the improvement of effect in clinical applications.

 摘要： [Objectives] This study aims to establish the quality standard of Fuzheng Wangan granules. [Methods] The qualitative identification of Radix Bupleuri and Radix Paeoniae Alba was carried out by the thin layer chromatography (TLC). The content of ginsenosides Rg1, Re and Rb was determined by the high performance liquid chromatography (HPLC). [Results] The spots of Radix Bupleuri and Radix Paeoniae Alba were clear, and the negative control had no interference. The total content of ginsenoside Rg1, Re and Rb ranged from 0.076 2 to 0.073 9 mg/g. [Conclusions] The method for the quality control of Fuzheng Wangan granules established in this study is stable and feasible.

 摘要： [Objectives] To investigate the optimal preparation process for lemon-flavored solid instant tea. [Methods] The formulation process was optimized through a single-factor combined orthogonal test, utilizing large-leaf Pu’er tea as the primary raw material and perfume lemon as the auxiliary ingredient. Sensory scores and the effectiveness of the spray drying process served as the key indicators for this investigation. [Results] The optimal formulation for lemon-flavored solid instant tea was established as follows: 15 g of Pu’er tea, 25 g of perfume lemon, a solid-liquid ratio of 1：20 (g/mL), an extraction temperature of 90 ℃, an extraction duration of 30 min, and air inlet and outlet temperatures of 200 ℃ and 80 ℃, respectively. This formulation produced the most favorable flavor profile for the perfume lemon-flavored solid instant tea, which was characterized by a stable texture and a sensory evaluation score of 91.97 points. [Conclusions] This study has the potential to enhance the development and utilization of large-leaf Pu’er tea and perfume lemon resources.

 摘要： [Objectives] To compare the leaf morphology and leaf venation patterns of Hedyotis auricularia L. and its adulterants, such as Mitracarpus hirtus (L.) DC., Spermacoce pusilla Wall. and Spermacoce alata Aubl., produced in Guangxi, so as to provide a simple and rapid identification method for the identification of H. auricularia L. in Guangxi. [Methods] LMVP (leaf morphology-venation pattern) identification method was used to study the characteristics of leaf morphology-venation pattern. [Results] Characteristics of leaf morphology-venation pattern: (i) H. auricularia L.: circular knotted-curved pinnate leaf venation without reaching the margin; the primary veins run straight without branches, the number of secondary veins is 5 to 9, and the angles included in tertiary veins are mostly near right angles or obtuse angles. (ii) M. hirtus (L.) DC.: curved pinnate leaf venation without reaching the margin; the primary veins run straight without obvious changes, the number of secondary veins is 3 to 5, and the tertiary veins are slender, and the included angles are mostly acute. (iii) S. pusilla Wall.: curved leaf venation without reaching the leaf margin; there are 4 to 5 pairs of secondary veins, most of which are opposite; the angle between the secondary veins and the primary veins in the middle and near the petiole is mostly medium acute angle, and the extension of the tertiary veins has no fixed direction. (iv) S. alata Aubl.: circular knotted-curved pinnate leaf venation; the primary veins are convex and straight without branches, and there are 4 to 6 pairs of secondary veins, alternating. The angle between secondary veins and primary veins is mostly medium acute angle, the angle between tertiary veins and secondary veins is near right angle, and the tertiary veins are mostly transversely extended. [Conclusions] The leaf morphology-venation patterns of the above-mentioned H. auricularia L. and M. hirtus (L.) DC., S. pusilla Wall., and S. alata Aubl. can be used as their identification features, and the identification method has certain operability, which provides an identification idea for the identification of Chinese herbal medicines.

 摘要： [Objectives] To study the effect of carcinoma cell line (HepG2) and mechanism of anti-tumor activity of cucurbitacin B (CuB) and cucurbitacin E (CuE) on HepG2 cells. [Methods] HepG2 cells were treated with various concentrations of CuB and CuE, and the proliferation, cell cycle distribution, apoptosis, and performed RNA-seqtranscriptomics of these cells were evaluated after treatment. [Results] The results demonstrated that, in comparison to the control group, HepG2 cells treated with CuB and CuE displayed a synergistic effect on growth inhibition, cell cycle arrest at the G₂/M phase, and apoptosis induction in a concentration- and time-dependent manner. Western blotting analysis using protein extracts derived from HepG2 cells showed that CuB and CuE induced apoptosis by increasing the expression of endogenous levels of full-length caspase-3 (35 kDa) and the large fragment of cleaved caspase-3 (17 kDa). Moreover, transcriptomic results demonstrated that after treatment, the differentially expressed genes associated with pathways such as "cell cycle", "spliceosome", "metabolic pathways", and "carbon metabolism" were significantly up-/down-regulated. [Conclusions] These results demonstrate that the anti-tumor mechanisms of CuB and CuE are attributed to their interference in cell cycle and metabolism processes. Collectively, the treatment with CuB and CuE may serve as a promising therapeutic option for hepatocellular carcinoma.