 摘要： 0

 摘要： [Objectives] To investigate the mechanism of action by which the traditional Chinese medicine (TCM) compound Cang Er Zi Nasal Relief patch alleviates nasal inflammation through network pharmacology. [Methods] The active ingredients and target sites of the Cang Er Zi Nasal Relief patch formulation were screened and collected through a comprehensive literature review and analysis of the TCMSP database. Target sites associated with nasal inflammatory diseases were obtained from the TTD, OMIM, and GeneCards databases. String database analysis was performed to identify common targets between active ingredients and diseases. Cytoscape software was used to construct network diagrams, facilitating the identification of core active ingredients and target molecules in the herbal patch formulation. Additionally, the DAVID database was employed to perform Gene Ontology (GO) functional analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis on the identified target molecules. [Results] The core herbal ingredients of the Cang Er Zi Nasal Relief patch may include Baical Skullcap Root, Centipeda minima, Divaricate Saposhnikovia Root, Cablin Potchouli Herb, Magnoliae Flos, and Menthae Herba. The core active ingredients likely include apigenin, luteolin, oxypeucedanin, emodin, rosmarinic acid, etc., which contribute to the alleviation of nasal inflammation. The core action targets may involve ESR2, TNF-α, AKT1, IL-4, MPO, and IL-6. The active ingredients in the health patch may alleviate nasal inflammation and associated symptoms like rhinorrhea, nasal itching, congestion, and dryness by targeting signaling pathways including IL-17, TNF, JAK-STAT, PI3K-Akt, and NF-κB within the body. [Conclusions] The clear and verifiable chain of action—“topical patches, transdermal absorption of active ingredients, regulation of core pathways, and alleviation of inflammatory symptoms”—provides robust molecular biological evidence supporting the clinical application of TCM in the external treatment of nasal disorders.

 摘要： [Objectives]To investigate the impact of storage duration on the DPPH free radical scavenging activity in Bauhinia championii honey and to assess its antioxidant activity. [Methods] The free radical scavenging activity of 38 batches of B. championii honey (fresh honey stage, aged honey stage I, aged honey stage II), was evaluated using the microplate DPPH assay. Honey sample solutions at varying concentrations (10, 25, 40, 55, and 70 g/L) were prepared, and 100 μL of each was combined with 100 μL of 0.05 g/L DPPH ethanol solution. The reaction mixtures were incubated at room temperature in the dark for 30 min. Subsequently, absorbance was measured at 517 nm, and both the scavenging rate and the half maximal inhibitory concentration (IC₅₀) were calculated. [Results] The IC₅₀ values for the DPPH free radical scavenging activity in 38 batches of B. championii honey at the fresh honey stage ranged from 8.96 to 58.65 mg/mL, with a mean value of 30.48 mg/mL. During the aged honey stage I, the IC₅₀ values ranged from 8.32 to 55.08 mg/mL, averaging 28.48 mg/mL. In the aged honey stage II, theIC₅₀ values ranged from 16.71 to 68.01 mg/mL, with a mean of 41.91 mg/mL. The methodological evaluation revealed that the relative standard deviations (RSD) for precision, repeatability, and stability were 0.02%, 2.21%, and 2.23%, respectively. [Conclusions] The DPPH free radical scavenging activity of 38 batches of B. championii honey remained robust throughout a 3-year storage period. However, it is advisable to limit the storage duration to within 2 years during production and storage to better preserve its antioxidant properties. The IC₅₀ value for DPPH free radical scavenging activity serves as a critical indicator for assessing the storage stability and freshness of B. championii honey.

 摘要： [Objectives] To investigate the uric acid-lowering effects of Clerodendranthus spicatus (Thunb.) C.Y.Wu ex H.W. Li. [Methods] After the optimization and scale-up of C. spicatus extraction, HP-20 macroporous resin was employed for fractionation using four eluents with ethanol concentrations of 10%, 40%, 70%, and 95%, yielding fractions designated as Fr.1 to Fr.4. The pharmacological effects of these fractions (Fr.1–Fr.4) were subsequently investigated in hyperuricemic model animals. [Results] Fr.1–Fr.4 reduced the serum levels of uric acid (UA), xanthine oxidase (XOD), blood urea nitrogen (BUN), and creatinine (CRE) in hyperuricemic rats, with Fr.4 demonstrating the optimal effect. All fractions increased body weight, decreased renal index, and exhibited certain protective effects against renal injury. [Conclusions] C. spicatus exhibits anti-hyperuricemic activity by reducing serum uric acid levels and also displays obvious renal protective effects.

 摘要： [Objectives] To investigate the acute and chronic toxic reactions of Dujieqing Pills and provide a basis for its safe clinical use. [Methods] The acute toxicity test employed the maximum dose tolerance test. The chronic toxicity experiment divided rats into low, medium, and high dose groups, receiving 10, 20, and 40 times the proposed clinical dose, respectively. Administration continued for 13 weeks, during which organ coefficients, histopathological changes, and toxicity reversibility were observed. [Results] In the acute toxicity experiment, the maximum tolerated gavage dose of Dujieqing Pills for KM mice was 84 g/(kg·d), with no significant toxic reactions observed at this dose. In the chronic toxicity experiment, SD rats administered the pills continuously for 13 weeks showed no significant abnormalities in appearance, behavioral activities, body weight, organ coefficients, or histopathological examinations. [Conclusions] No significant toxic reactions were observed in either the acute or chronic toxicity tests, suggesting that Dujieqing Pills exhibit good safety for single or long-term use at the proposed clinical dose.

 摘要： [Objectives] To optimize key fresh-cut technologies for mulberry branches by employing multiple evaluation indicators and to compare their antioxidant capacity with that of traditionally processed slices, thereby providing guidance for the fresh-cut processing of mulberry branch medicinal materials. [Methods] A single-factor test method was employed to examine the effects of the drying degree of mulberry branches prior to cutting, cutting thickness, drying method, and drying temperature on the quality of slices. Evaluation indicators included appearance characteristics, alcohol extract content, total polysaccharide content, and total flavonol content, which were used to determine the optimal fresh-cut processing conditions. Additionally, the in vitro antioxidant activity of the samples was assessed using the DPPH free radical scavenging assay. [Results] The optimal parameters for fresh-cut processing of mulberry branches were identified as follows: a moisture content of (42±5)% prior to cutting, a slicing thickness of 4 mm, and hot air drying at 70 °C. Slices produced under these conditions exhibited a smooth appearance, with relatively high levels of alcohol extract, total polysaccharides, and total flavonoids. Antioxidant activity assessment revealed that the IC₅₀value of the fresh-cut processed slices against DPPH free radicals was 5.61 mg/mL, demonstrating superior efficacy compared to traditionally processed slices, which had an IC₅₀ value of 6.85 mg/mL. [Conclusions] The optimized fresh-cut process for mulberry branches is both reasonable and feasible, demonstrating high repeatability. This process significantly enhances the quality of slices and the retention of active components, and improves antioxidant activity, thereby offering a scientific foundation and practical guidance for the initial processing of mulberry branches at their source.

 摘要： [Objectives] To establish a high-performance liquid chromatography (HPLC) method for comparing the content differences of xanthopurpurin in raw Rubia cordifolia and its charred products processed at different temperatures, sourced from various regions. [Methods] The chromatographic separation was performed using an HPLC COLUMN C₁₈. The mobile phase consisted of acetonitrile-0.2% phosphoric acid solution (50:50, v/v) at a flow rate of 1.0 mL/min. Detection was carried out at 245 nm, and the column temperature was maintained at 30 ℃. [Results] Xanthopurpurin exhibited a good linear relationship within the range of 6.712～83.905 μg/mL (r=1.000 0). The average recovery rate was 101.3% with an RSD of 2.1% (n=6). Content determination results showed that the xanthopurpurin content in raw R. cordifolia ranged from 0.040 0 to 0.169 3 mg/g. In charred R. cordifolia processed at 110-130 ℃, it ranged from 0.309 2 to 0.380 1 mg/g, while in charred R. cordifolia processed at 350-370 ℃, it ranged from 0.512 8 to 1.168 8 mg/g. A significant increasing trend in xanthopurpurin content was observed with rising charring temperatures. [Conclusions] This method is simple, accurate, feasible, and reproducible. It can effectively distinguish between raw R. cordifolia and its charred products processed at different temperatures, providing a scientific basis for the quality evaluation of charred R. cordifolia and its formulations in traditional Chinese medicine.

 摘要： [Objectives] To screen the optimal formulation process for Zhuang medicine Yishanhong Granules. [Methods] An L₉(3⁴) orthogonal design was employed to optimize the granulation process, with comprehensive assessment based on the granule yield, moisture absorption, flowability, and color uniformity. The factors investigated included the amount and ratio of excipients, and the mesh size of the sieve. [Results] The optimized parameters were as follows: a 3:2 ratio of dextrin to mannitol as excipients, a 1:3 ratio of extract to excipients, and a 14-mesh sieve. Under these conditions, Zhuang Medicine Yishanhong Granules were successfully obtained. [Conclusions] The optimal formulation process for Zhuang medicine Yishanhong Granules was determined. The granules prepared under these conditions exhibited a satisfactory formation rate, along with favorable properties in terms of moisture absorption, flowability, dispersibility, and color uniformity. This process is rational and feasible, thereby providing a reliable basis for industrial production.

 摘要： [Objectives] To investigate the mechanism by which Dachaihu Decoction affects visceral pain behavior in a rat model of ulcerative colitis (UC). [Methods] Twenty-one male Sprague-Dawley (SD) rats were randomly and equally divided into a control group, a UC group, and a Dachaihu Decoction group (n=7 per group). After treatment completion, visceral pain behavior and colonic motility indices were assessed using in vivo colorectal distension (CRD) and abdominal withdrawal reflex (AWR) tests. [Results] Compared to the UC group, the Dachaihu Decoction group exhibited increased colon length, elevated colonic basal tension, heightened peak contractile tension, and increased contraction frequency. These differences were statistically significant (P<0.05). [Conclusions] Dachaihu Decoction may ameliorate visceral hyperalgesia induced by rectal distension stimuli in the UC rat model by improving colonic motility.

 摘要： [Objectives] To analyze the application effect of failure mode and effect analysis (FMEA) in the management of ECMO emergency treatment procedures in children. [Methods] The FMEA project team was established, the theme was determined, the workflow diagram of ECMO was drawn, the failure mode of the emergency treatment process management of children was determined, the root cause of the failure mode was analyzed, and improvement measures were formulated and implemented. [Results] Compared with before the improvement, the time of ECMO personnel in place, item preparation time, pipe prefilling time and bedside catheterization time were significantly shortened (all P<0.05), and the satisfaction of medical and nursing cooperation and parents of the children were significantly increased (all P<0.05). [Conclusions] FMEA can optimize the emergency treatment management process of ECMO, shorten the start time of ECMO, improve medical cooperation and parental satisfaction, and help improve the success rate of ECMO treatment in children.