 摘要： [Objectives] To establish a microbial limit test method for Wuteng Qufeng Zhitong Powder, ensuring its compliance with the relevant requirements of the Chinese Pharmacopoeia 2020 Edition. [Methods] In accordance with the Chinese Pharmacopoeia 2020 Edition, General Chapters Part IV, 1105 and 1106, method suitability tests were conducted for the total aerobic microbial count (TAMC), total yeasts and molds count (TYMC), and for the specified microorganisms (Staphylococcus aureus, Pseudomonas aeruginosa). Representative test strains, including Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Candida albicans, and Aspergillus niger, were employed in spiked recovery tests using the pour plate method to calculate the recovery ratio. Validation was performed on three batches of the product. [Results] The recovery ratios for all test strains fell within the range of 0.5 to 2.0. In the specified microorganism tests, the corresponding strains were successfully detected in the test groups, indicating that the established test sample preparation method effectively neutralized the sample's potential antimicrobial activity. The microbial limit test results for all three batches complied with the specifications. [Conclusions] The established microbial limit test method is accurate, reliable, and suitable for the microbiological quality control of Wuteng Qufeng Zhitong Powder.

 摘要： [Objectives] To determine the heavy metal content in lapis lazuli (complex processing) and provide a reference for establishing its heavy metal limit standards. [Methods] Seven batches of lapis lazuli (complex processing) samples were collected from Hongyuan County Tibetan Medicine Hospital, Aba Tibetan and Qiang Autonomous Prefecture Tibetan Medicine Hospital, and Aba County Tibetan Medicine Hospital. The contents of Hg, As, Pb, Cd, and Cu were determined using inductively coupled plasma mass spectrometry in accordance with General Rule 2321 of the Pharmacopoeia of the People's Republic of China (2020 Edition). Tests for heavy metal limits and arsenic salt limits were conducted according to General Rules 0821 and 0822, respectively. [Results] The Cu content in the seven batches of samples ranged from 8.49 to 21.41 mg/kg, As content ranged from 6.82 to 16.87 mg/kg, Hg content ranged from 0.03 to 0.62 mg/kg, and Pb content ranged from 3.66 to 26.98 mg/kg. Cd was not detected in any of the samples. The contents of heavy metals varied considerably among samples from different origins. The heavy metal limit test yielded a result of ≤50 mg/kg, while the arsenic salt limit test produced a result of ≤40 mg/kg. [Conclusions] The heavy metal content in various processed lapis lazuli products varies significantly. Processing technology is an important factor influencing the heavy metal content. This study provides fundamental data for the development of processing specifications and quality standards for lapis lazuli.

 摘要： [Objectives] To address the issues of rapid deterioration and unfavorable taste in compound honeysuckle preparations, as well as the challenges of high viscosity, low efficiency, and elevated water absorption rates associated with the current film coating process, optimize the process and offer a reference framework for industrial enhancement. [Methods] Utilizing a literature review approach, the current research status of process is systematically examined. The challenges associated with the application and industrialization of multi-dosage forms are analyzed, and improvement strategies are proposed across five domains: materials, parameters, equipment, quality control, and fundamental research. [Results] This process can improve the stability of preparations, mask odors, and be adapted for the production of various dosage forms. However, it presents challenges including fluctuation in the quality of plain tablets, uneven coating application, and inadequate standardization. Systematic optimization can effectively address these process deficiencies. [Conclusions] Following enhancements in greenness, intelligence, and standardization, the compound honeysuckle film coating process demonstrates extensive application potential and offers valuable insights for advancing coating technologies and promoting the industrial upgrading of solid preparations in traditional Chinese medicine.

 摘要： [Objectives] To establish a quality standard for pomelo (Citrus maxima) peel. [Methods] According to the relevant methods specified in the General Chapters of the Chinese Pharmacopoeia (2020 Edition, Volume IV), ten batches of pomelo peel from different producing areas were examined for moisture, total ash, acid-insoluble ash, and extractives. A thin-layer chromatography (TLC) identification method was established using naringin as the marker, and high-performance liquid chromatography (HPLC) was employed for the content determination of naringin. [Results] Microscopic examination revealed characteristic structures of the Citrus genus, such as calcium oxalate prism crystals, vessels, and oil cells. The TLC spots were clear and well separated without interference from the negative control. For the ten batches of samples, the moisture content ranged from 9.8% to 12.7%, total ash from 3.1% to 5.1%, acid-insoluble ash from 0.04% to 0.2%, water-soluble extractives from 44.1% to 57.2%, ethanol-soluble extractives from 31.4% to 44.6%, and naringin content from 1.65% to 7.63%. [Conclusions] The established quality standard method is accurate, reliable, and reproducible, and can be applied to the quality control of pomelo peel.

 摘要： Combretastatin is a natural active substance isolated from the bark of South Africa Combretum alfredii, which has pharmacological activities such as anti-tumor, anti-inflammatory, antifungal, and immunomodulation. In terms of anti-tumor effects, it exerts the effects by inhibiting tumor cell proliferation, inducing apoptosis, and suppressing angiogenesis. The mechanism involves activation of the caspase family and regulation of the VEGF/VEGFR-2 and Notch signaling pathways. In terms of anti-inflammatory effects, it inhibits the release of pro-inflammatory factors such as TNF-α and IL-6 by blocking the NF-κB pathway. In terms of antifungal effects, it targets fungal microtubule proteins and induces microtubule depolymerization. In terms of immunomodulation, it promotes the proliferation of immune cells and regulates M1 polarization of macrophages. In this paper, the core pharmacological effects and molecular mechanisms of combretastatin are reviewed, providing theoretical references for its new drug development.

 摘要： [Objectives] To systematically screen the core active components, action targets, and signaling pathways of Jiebai Tongfei Zhike Patch based on network pharmacology, and to elucidate its multi-component, multi-target, and multi-pathway synergistic mechanism in the external transdermal treatment of cough. [Methods] Active components and corresponding targets were screened using the TCMSP database and literature retrieval, with further selection based on oral bioavailability, drug‑likeness indices, and transdermal absorption characteristics. Disease‑related targets were obtained from the TTD, OMIM, and GeneCards databases. A protein‑protein interaction (PPI) network and a “Chinese herbal medicine–active component–target” network were constructed using String and Cytoscape. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed via DAVID. [Results] A total of 125 active components were screened, corresponding to 405 targets; 430 disease‑related targets were obtained, and 42 overlapping targets were identified. The core Chinese medicinal herbs were Glycyrrhiza uralensis (Gancao), Stemona sessilifolia (Baibu), Lonicera japonica (Jinyinhua), and Saposhnikovia divaricata (Fangfeng). The core active components included lipophilic small‑molecule compounds such as luteolin, apigenin, and epigallocatechin gallate. The core targets included PTGS2, TNF, IL6, AKT1, JUN, and TP53. GO analysis revealed involvement in processes such as transcriptional regulation, negative regulation of apoptosis, and immune response. KEGG enrichment was mainly associated with signaling pathways including PI3K‑Akt, JAK‑STAT, TNF, and MAPK. [Conclusions] Through the synergistic effects of multi‑component, multi-target, and multi-pathway interactions, Jiebai Tongfei Zhike Patch inhibits inflammatory responses, reduces cough sensitivity, and modulates immune function, thereby exerting the therapeutic actions of moistening the lung and relieving cough, regulating qi and resolving phlegm, and ventilating the lung and calming wheezing.

 摘要： [Objectives] To explore the mechanisms of Jianzhong Chubi Decoction in treating comorbid knee osteoarthritis (KOA) and chronic gastritis (CG) based on network pharmacology and molecular docking approaches. [Methods] Active components and their corresponding targets of Jianzhong Chubi Decoction were retrieved from the TCMSP and SymMap databases. Disease targets for KOA and CG were collected from GeneCards. Overlapping targets were used to construct a “drug–active component–target” network and a protein-protein interaction (PPI) network. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed. Molecular docking was subsequently conducted to validate the binding affinities between the core active components and key targets. [Results] A total of 104 active components and 440 targets were obtained for Jianzhong Chubi Decoction, along with 1 500 KOA targets and 1 000 CG targets, yielding 98 intersecting targets. The core components were quercetin, kaempferol, and feruloyltyramine; the key targets included TNF, AKT1, IL-6, and IL-1β. KEGG enrichment analysis mainly involved cancer pathways, lipid and atherosclerosis pathways, and the AGE-RAGE signaling pathway. Molecular docking showed that the binding energies of the core components with the key targets were all below −5 kcal/mol. [Conclusions] Jianzhong Chubi Decoction exerts anti-inflammatory, cartilage-protective, and gastric mucosa-protective effects through multiple components, multiple targets, and multiple pathways. This provides a pharmacological basis for the concept of “treating different diseases with the same therapy” in KOA and CG comorbidity.

 摘要： This paper review summarizes the analgesic, anti-tumor, antiarrhythmic, anti-inflammatory, and CNS inhibitory activities of oxysophoridine, along with their underlying molecular mechanisms. In terms of analgesic activity, oxysophoridine exerts pain‑relieving effects by enhancing GABAergic inhibitory function. Regarding anti-tumor activity, its combination with salvianolic acid A can synergistically block the cell cycle, induce apoptosis, and inhibit the proliferation of cervical precancerous cells. With respect to antiarrhythmic activity, it acts through non‑specific antagonism of the adrenergic system and blockade of sodium ion influx. As for anti‑inflammatory activity, it downregulates CD14/TLR4 expression, inhibits pathways such as NF‑κB, and reduces the release of inflammatory cytokines. Furthermore, in its CNS inhibitory activity, it dose‑dependently suppresses spontaneous locomotor activity in mice and synergistically enhances hypnotic effects. This review provides a theoretical basis for the further development and clinical application of oxysophoridine.

 摘要： [Objectives] To observe the protective effect of Danhong Decoction on cerebral ischemia‑reperfusion injury in Sprague-Dawley (SD) rats. [Methods] Eighteen SD rats were randomly divided into blank control group, MCAO group, and observation group (n=6 each). In the MCAO and observation groups, the cerebral ischemia‑reperfusion model was established by the suture occlusion method (ischemia for 60 min followed by reperfusion). After successful modeling, the observation group received 2% Danhong Decoction (2 mL/rat) by intragastric administration twice daily for 1 week, while the blank control and MCAO groups received an equal volume of normal saline. After treatment, the expression levels of endothelial nitric oxide synthase (eNOS), nitric oxide (NO), malondialdehyde (MDA), superoxide dismutase (SOD), vascular endothelial growth factor (VEGF), and endothelin‑B (ET‑B) receptor in brain tissue were measured. [Results] Compared with the MCAO group, the observation group showed significantly decreased levels of MDA, ET‑B protein, and ET‑B mRNA, and significantly increased levels of NO, eNOS, SOD, VEGF, and VEGF mRNA (all P < 0.05). [Conclusions] Danhong Decoction can attenuate cerebral ischemia-reperfusion injury in SD rats, and its mechanism may be related to inhibition of oxidative stress and promotion of angiogenesis.

 摘要： [Objectives] To investigate the active ingredients and targets of Guanxin No. 3 formula in the treatment of myocardial infarction (MI) through the application of network pharmacology and molecular docking techniques. [Methods] The active ingredients and corresponding targets of Guanxin No. 3 Formula were identified using the TCMSP database, while MI targets were retrieved from the GeneCards and OMIM databases. Following the identification of overlapping targets, a “drug-active ingredient-target” network was constructed. A protein–protein interaction (PPI) network was subsequently established utilizing the STRING database. GO and KEGG enrichment analyses were performed via the DAVID platform. Molecular docking validation was conducted employing AutoDock Vina and PyMOL software. [Results] A total of 124 active ingredients were identified, corresponding to 190 MI-related targets. The key targets included IL6, AKT1, TP53, TNF, IL1B, etc. KEGG pathway enrichment analysis mainly involved lipid and atherosclerosis, and signaling pathways such as TNF, IL-17, HIF-1, and PI3K-Akt. Molecular docking results demonstrated that key ingredients, including luteolin, quercetin, and β-sitosterol, exhibited strong binding affinities with these key targets. [Conclusions] Guanxin No. 3 Formula may exert anti-MI effects by regulating inflammatory responses, inhibiting apoptosis, and promoting angiogenesis via multiple ingredients and targets.