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 摘要： [Objectives] To quantify the content of ceftazidime-avibactam (CAZ-AVI) employing green analytical chemistry principles and assess the environmental sustainability of the proposed method. [Methods] The concentrations of disodium hydrogen phosphate and potassium dihydrogen phosphate, as well as the pH of the buffer solution, were optimized using a Box-Behnken design. The evaluation method's performance was validated through methodological assessment, and its environmental sustainability was appraised employing the AGREE, BAGI, and Complex GAPI tools. [Results] The optimal conditions were determined to be 27.79 mmol/L disodium hydrogen phosphate, 20.27 mmol/L potassium dihydrogen phosphate, and a pH of 3.52. The linear ranges for ceftazidime (CAZ) and avibactam (AVI) were 0.09–2.25 and 0.1–3 μg/mL, respectively, with correlation coefficients exceeding 0.999. The respective contents were 87.61% and 9.90%. Recovery rates were 98.56% for CAZ and 97.57% for AVI, while the detection limits were as low as 0.44 and 0.75 ng/mL, respectively. The green chemistry evaluation yielded a favorable result, with an AGREE score of 0.85. [Conclusions] The method is sensitive, accurate, and environmentally sustainable, making it suitable for the quality inspection of CAZ-AVI.

 摘要： [Objectives] To analyze the gene expression networks across distinct tissues of Nardostachys jatamansi and elucidate the correlation between key gene expression levels in leaves and nardosinone accumulation in medicinal rhizomes, providing a technical foundation for rapid monitoring of bioactive compound accumulation.[Methods] Transcriptome sequencing coupled with Weighted Gene  Co-expression Network Analysis (WGCNA) was employed to construct gene co-expression networks, Ultra-performance Liquid Chromatography (UPLC) was utilized to quantify nardosinone content across various growth stages. Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) was integrated to validate spatiotemporal relationships between key gene expression profiles and nardosinone accumulation patterns. [Results] Among seven co-expression modules, the blue module demonstrated an exceptionally strong correlation with nardosinone content (r=0.993, p=1.2×10⁻¹⁵). This module encompassed pivotal genes such as NjDXS3 and NjTPS22. Three-year-old N. jatamansi plants reached peak nardosinone accumulation in September, with leaf expression levels of NjDXS3 closely mirroring this accumulation trend, whereas NjTPS22 expression exhibited minimal temporal variation. A critical threshold ratio exceeding 4.5 for NjDXS3/NjTPS22 expression in leaves signaled peak nardosinone concentrations. [Conclusions] By monitoring the expression ratio of NjDXS3 to NjTPS22 in leaves, the accumulation status of bioactive compounds in medicinal organs can be non-invasively assessed, offering a novel methodology for quality evaluation of N. jatamansi crude drugs.

 摘要： [Objectives] To optimize the extraction and preparation processes of Guichen Xuanfei Mixture and establish a foundation for its industrial-scale production. [Methods] Using the dry paste rate as the evaluation index, a single-factor experiment was conducted to examine the effects of soaking time, volume of water added, and extraction duration. Subsequently, orthogonal experiments were employed to optimize the volume of water added, extraction duration, and extraction frequency. Following the determination of the optimal extraction process, process validation tests and pilot-scale production were performed. [Results] The optimal extraction process involved soaking the medicinal materials in water for 30 min, followed by two successive boiling steps. During the first extraction, 8 times the volume of water relative to the material was used, and 6 times the volume was applied during the second extraction. Each extraction was conducted for 1 h. The average dry paste rate obtained from the verification test was 24.25%, while the yield rates for the three batches of pilot-scale production were 93.6%, 93.9%, and 93.6%, respectively. [Conclusions] The optimized extraction and preparation method is rational, stable, and demonstrates excellent reproducibility, making it well-suited for the industrial-scale production of Guichen Xuanfei Mixture. 

 摘要： [Objectives] To study the process of Zhuang medicine Wuzhi Maotao Buxu Granule. [Methods] The orthogonal design method was used to optimize the best water extraction process with the amount of water, extraction time and extraction times as factors and the dry extraction yield of granules as the indicators. The concentration process was screened and molding process and the pilot production were studied. [Results] The optimal water extraction process was as follows: soaked in water for 30 min, decocted twice—first with 10 times the amount of water for 1 h, then with 8 times the amount of water for 1 h. The extract was concentrated using a two-step method: initial concentration under reduced pressure, followed by secondary concentration at atmospheric pressure. Ethanol concentration was 85%, with dextrin as the filler. The product yield of three batches of Wuzhi Maotao Buxu Granule all exceeded 80%. [Conclusions] Wuzhi Maotao Buxu Granule was extracted using the water extraction method. Through the selection of concentration processes, research on granulation techniques, and pilot-scale production studies, the granules formed well with high yield, good fluidity, high efficiency, and suitability for large-scale production

 摘要： By reviewing domestic and international literature, a systematic summary on the pharmacological effects and molecular mechanisms of isolinderalactone (Iso) was conducted. The results indicate that Iso mitigates Aβ42-induced neuronal damage by inhibiting the JNK signaling pathway, exerting an anti-Alzheimer's disease effect. Iso specifically inhibits Helicobacter pylori. By activating the LXRα pathway, Iso suppresses polarization of macrophage M1 and reduces production of inflammatory mediator, demonstrating anti-ulcerative colitis activity. By inducing ROS accumulation, regulating mitochondrial apoptosis pathways, and activating the MAPK pathway, Iso inhibits the cell proliferation of lung cancer, breast cancer, and colorectal cancer. Iso exhibits multi-target pharmacological activity and holds promise for applications in neurodegenerative diseases, infections, inflammation, and cancer prevention and treatment. 

 摘要： This paper provides a systematic review of the anti-tumor, anti-inflammatory, antibacterial, and anti-echinococcosis effects of harmine, as well as its role in regulating osteogenic differentiation and protecting the central nervous system, along with the underlying molecular mechanisms. Harmine exhibits anti-tumor properties by inhibiting the proliferation of gastric cancer, lung cancer, and other cancer cell types, inducing apoptosis, and impeding migration and invasion through pathways such as PTEN/AKT/MDM2. Regarding its anti-inflammatory and antibacterial effects, harmine reduces inflammation by modulating the NF-κB/NLRP3 pathway and exerts antibacterial activity by disrupting bacterial structures. Furthermore, harmine regulates the Th1/Th2 balance to produce anti-echinococcosis effects, promotes osteogenic differentiation by mediating the antioxidant activity of CA-9, and ameliorates anxious behavior through the regulation of neuroinflammation. This review offers a theoretical foundation for the further development and clinical application of harmine.

 摘要： [Objectives] To investigate the effects of Ardisia gigantifolia extract on oxidative stress and apoptosis in pancreatic cancer AsPC-1 cells. [Methods] MTT assay was used to detect the inhibitory effects of different concentrations (0, 2, 4, 6, 8, 10 μg/mL) of A. gigantifolia extract on the proliferation of AsPC-1 cells after 48 h treatment, and the half maximal inhibitory concentration (IC50) was calculated; Transwell assay was employed to evaluate the effects of the extract on cell migration and invasion; intracellular superoxide dismutase (SOD) activity and malondialdehyde (MDA) level were measured using commercial kits to reflect the oxidative stress status. [Results] A. gigantifolia extract significantly inhibited the proliferation of AsPC-1 cells (IC50 = 5.805 μg/mL), reduced their migration and invasion abilities, enhanced SOD activity, and decreased the oxidative stress level. [Conclusions] A. gigantifolia extract enhances SOD activity in pancreatic cancer AsPC-1 cells, reduces ROS levels, and induces apoptosis, thereby exerting an inhibitory effect on the viability of AsPC-1 cells.

 摘要： [Objectives] To establish a characteristic chromatogram of the traditional Yao medicine Kadsura longipedunculata, develop a method for quantifying its primary component, anwulignan, and evaluate the anti-inflammatory activity of anwulignan. [Methods] Gradient elution was performed using high-performance liquid chromatography (HPLC) with a mobile phase consisting of acetonitrile and 0.5% phosphoric acid solution. A characteristic chromatogram of K. longipedunculata was established, and its similarity was assessed using the Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (2012 edition). Additionally, the content of anwulignan in K. longipedunculata was quantified. Lipopolysaccharide-induced RAW264.7 macrophages were employed as an inflammatory cell model to investigate the effects of anwulignan on the levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6) in the cell culture supernatant. [Results] The similarity indices of characteristic chromatograms for 10 batches of K. longipedunculata ranged from 0.901 to 0.994, with 9 common peaks identified. Among these, three components were characterized, including changnan schisantherin E, kadsulactone A, and anwulignan. The content of anwulignan was quantified as ranging from (0.72 ± 0.05) to (1.21 ± 0.03) mg/g (n = 3). Furthermore, anwulignan at concentrations of 0.125–0.5 μg/mL significantly reduced the levels of TNF-α, IL-1β, and IL-6 in the supernatant of inflammatory model cells (P < 0.05 or P < 0.01). [Conclusions] HPLC characteristic chromatogram of K. longipedunculata and the method for determining the content of anwulignan have been established. Anwulignan may represent the active ingredient responsible for the anti-inflammatory effects observed in K. longipedunculata.

 摘要： [Objectives] To systematically investigate the high-efficiency intelligent facility cultivation techniques for Hericium erinaceus. [Methods] A three-layer intelligent regulation system encompassing "perception–execution–control" was established to enable precise monitoring and stage-specific automatic adjustment of key environmental parameters, including temperature, humidity, CO2 concentration, and light intensity. Combined with integrated green pest management practices, the effects of intelligent cultivation on mycelial growth, yield, marketable product rate, contamination rate, and overall economic benefits were systematically evaluated. [Results] Compared with traditional facility cultivation, the intelligent cultivation system shortened the time to full mycelial colonization of the substrate bag by 2–3 days, increased the average yield per artificial log by over 30%, achieved a marketable product rate of 92%, reduced the contamination rate to 2.23%, and significantly decreased labor input, resulting in an overall economic benefit increase exceeding 30%. [Conclusions] This technical system effectively addresses the production bottlenecks posed by high humidity and large temperature fluctuations during spring in the Changshan region, thereby achieving stable, high-quality, and efficient cultivation of H. erinaceus. The system demonstrates considerable feasibility for extension and application in regions with similar climatic conditions across the South Yangtze River area and provides a technical reference for the digital and intelligent transformation of the traditional edible mushroom industry.