Isolation and Identification of Rhizosphere Microorganisms and Endophytes in Pogostemon cablin
摘要:
[Objectives] To systematically investigate the microbial community composition of rhizosphere soil and endophytes associated with Pogostemon cablin, and to explore the relationships between endophytes and rhizosphere microorganisms as well as their potential applications. [Methods] Microbial isolates were obtained from rhizosphere soil, root tissues, and stem tissues using the serial dilution and spread plate method. These isolates were identified through morphological characterization, physiological and biochemical assays, and molecular biological techniques. [Results] A total of 18 microbial strains were isolated, including 7 bacterial and 11 fungal strains. Among the bacterial isolates, Pseudomonas spp. and Bacillus spp. were predominant, while the fungal isolates were mainly represented by Aspergillus spp. Certain bacterial strains, notably Pseudomonas spp., exhibited potential abilities for indole-3-acetic acid (IAA) production, nitrogen fixation, and antagonistic activity against pathogenic microorganisms, suggesting their potential utility as biocontrol agents and promoters of plant growth. [Conclusions] This study establishes a foundational understanding of the microbial community characteristics in the rhizosphere and tissues of P. cablin, as well as their roles in plant growth and development.
关键词:
Pogostemon cablin, Rhizosphere microorganism, Endophyte, Separation and identification