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 摘要： [Objectives] To develop methods for the early and rapid detection of tomato gray mold. [Methods] Utilizing the ACTIN gene of Botrytis cinerea as the target, a set of specific primers for loop-mediated isothermal amplification (LAMP) was designed and screened. Subsequently, the reaction system and conditions were optimized to achieve rapid isothermal amplification of B. cinerea. [Results] Through agarose gel electrophoresis and SYBR Green I visualization analysis, the optimal dosages of Bst II DNA polymerase and dNTPs, as well as the optimal ratio of internal to external primers, were determined to be 0.6 U/μL, 1.25 mmol/L, and 2：1, respectively. The specific detection of B. cinerea was accomplished at 61 ℃ for 40 min, achieving a sensitivity of 100 ag/μL, which is 10⁶ times greater than that of conventional PCR detection. When this method was applied to the detection of tomato diseases, the detection limit for B. cinerea spores reached 20 spores/mL. Furthermore, the pathogen was detectable in tomato leaves that had been infected for 4 d, despite the absence of visible phenotypic symptoms of gray mold. [Conclusions] This method is suitable for the early, rapid, sensitive, and visual detection of tomato gray mold, thereby offering technical support for its early diagnosis, prevention, and control.

 摘要： [Objectives] To assess the effectiveness of the intelligent small insect monitoring and forecasting system developed by Zhejiang Top Cloud-Agri Technology Co., Ltd. in monitoring, providing early warnings, and identifying rice planthoppers. [Methods] In 2024, an experiment involving the automatic identification and counting of rice planthoppers was conducted using the intelligent small insect monitoring and forecasting system in the rice production demonstration area of Qingxichang Sub-district, Xiushan Autonomous County, Chongqing City. The results obtained were subsequently compared and analyzed against those derived from manual identification. [Results] The intelligent small insect monitoring and forecasting system achieved recognition accuracy rates of 95.14%, 94.25%, and 97.78% for Nilaparvata lugens, Sogatella furcifera, and Laodelphax striatellus, respectively, resulting in an average accuracy rate of 95.72%. The outcomes derived from automatic recognition closely corresponded with those obtained through manual identification. [Conclusions] This research provides a reference for the optimization of the intelligent small insect monitoring and forecasting system.

 摘要： This paper reviews the historical application, primary species, and efficacy of predatory ants in pest management, and systematically elucidates their core mechanisms, including direct predation, non-lethal deterrence, and ecological regulation, as well as two application models: forest land introduction and farmland conservation. Meanwhile, the negative impacts on other arthropods, population diffusion, and management challenges in current applications are analyzed. Future research directions, such as precise assessment and risk control, integrated pest management (IPM) strategies integrating multiple technologies, and applications of molecular ecology, are clarified. The aim is to provide a reference for the in-depth research, promotion, and application of this type of natural enemy insect in sustainable IPM of agriculture and forestry.

 摘要： [Objectives] To identify the optimal combination of herbicide concentrations appropriate for soil closed weeding in the soybean and corn intercropping system in Zhongwei City. [Methods] Two herbicides, 96% (S)-metolachlor and 75% thifensulfuron methyl, were selected for this experiment. A no-herbicide treatment served as the control, and five concentration gradients of the herbicides were established. Prior to sowing, the soil underwent a closed weeding treatment. The impacts of various herbicide concentration combinations on growth indicators, weed control effect, and the yields of soybean and corn across various treatments were analyzed. [Results] The tested combinations of herbicide concentrations did not result in significant phytotoxicity to soybean and corn seedlings. Furthermore, as the herbicide concentrations increased in each treatment, treatments D and E demonstrated the most effective weed control effect. Specifically, 40 d post application, the plant control effect and fresh weight control effect reached 97.25% and 98.03% for treatment D, and 97.25% and 98.24% for treatment E, respectively. Additionally, the yields of both soybean and corn showed significant increases. [Conclusions] Considering the overall output-input ratio in this region, treatment D, comprising 96% (S)-metolachlor at 1 650 mL/hm² and 75% thifensulfuron-methyl at 48 g/hm2, can be identified as the herbicide concentration combination that provides the most effective weed control effect in the soybean and corn strip intercropping system.

 摘要： Based on a comprehensive inspection and acceptance evaluation of the biological firebreak forest belt construction project in Huiyang Forest Area, this study systematically analyzes common issues encountered during the acceptance process. The findings indicate that primary challenges include low seedling survival rates, incongruities between tree species selection and site conditions, structural deficiencies within the forest belts, insufficient post-planting maintenance and management, and irregularities in documentation. To address these problems, the study proposes targeted countermeasures, such as optimizing tree species selection, enhancing supervision of planting techniques, and establishing a long-term ecological compensation-based management model. These recommendations are framed within the contexts of scientific planning, precise implementation, strengthened oversight, and innovative management mechanisms. The research aims to improve the construction quality and long-term fire prevention efficacy of biological firebreak forest belts, thereby providing practical guidance for analogous firebreak forest belt construction and acceptance initiatives in forest areas of South China.

 摘要： Through on-site investigations and case analyses of green mines in Huizhou City, and by integrating technical measures including soil improvement, plant configuration, and community construction, this study proposes a plant configuration model tailored for ecological restoration in the mining areas of Huizhou. The implementation of a multi-level configuration model that predominantly employs native plant species, combined with a "soil-vegetation-microorganism" collaborative restoration strategy, can significantly enhance vegetation coverage in mining areas, increase soil organic matter content, and reduce the bioavailability of heavy metals in the soil. Based on field research and case study analyses, several optimization recommendations are proposed from the perspectives of slope stability and soil and water conservation. Ultimately, it summarizes plant configuration models and their application effects in the ecological restoration of green mines in Huizhou City. These findings may serve as valuable references for the ecological restoration of mines in South China.

 摘要： [Objectives] To investigate the effects of silicon fertilizer spraying on the growth, yield, quality, and overall benefits of rice cultivation. [Methods] A systematic experiment involving the single-spray multi-promotion technology of silicon fertilizer via unmanned aerial vehicles (UAVs) was conducted in three representative rice-growing areas: Ma’an Town, Shuikou Subdistrict, and Luzhou Town. [Results] The spraying of silicon fertilizer markedly enhanced the root development of rice, resulting in increased tiller number, plant height, stem thickness, panicle length, and 1 000-grain weight, thereby effectively improving both yield and quality. This treatment exerted six primary beneficial effects: promoting robust and stable seedling growth, enhancing stress resistance, reducing reliance on chemical fertilizers, improving quality, increasing economic benefits, and significantly advancing ecological and social benefits. [Conclusions] The application of silicon fertilizer through spraying is an effective agronomic practice that simultaneously promotes increased rice yield, improved quality, enhanced efficiency, and the sustainable development of resources and the environment.

 摘要： Using the curriculum of horticultural plant pathology as a starting point, this paper investigates the collaborative education model of "curriculum ideology and politics+innovation and entrepreneurship". It expounds the necessity of constructing this model, proposes implementation paths from aspects such as the integration of ideological and political elements, the cultivation of innovation and entrepreneurship capabilities, and the construction of a collaborative education mechanism. Furthermore, it analyzes the practical effects, challenges encountered, and corresponding response strategies, thereby offering a valuable reference for the training of professionals in horticulture.

 摘要： Within the context of the rural revitalization strategy and the construction of ecological civilization, this study examines the village-level forest park construction project in Xijiao Village, Henghe Town, as an empirical case to investigate its planning, design, and practical implementation pathways. Drawing upon the distinctive natural landscape and local cultural assets of Xijiao Village, the research systematically addresses ecological conservation, functional zoning, and landscape development through vegetation transformation and infrastructure development, thereby comprehensively enhancing regional landscape quality and recreational functions. The project exemplifies that scientifically grounded and rational planning and construction can substantially improve rural living environments, foster the growth of rural ecotourism, and offer replicable planning models and practical insights for the development of village-level forest parks in comparable regions.