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Preliminary Screening of Agents for the Management of Root Rot in Tobacco Floating Seedlings
摘要: [Objectives] To screen effective agents for the management of root rot in tobacco floating seedlings. [Methods] A total of eight treatments were implemented in a large-scale field trial, which included three replications. Each treatment was administered at the recommended dosages. [Results] The application of Metalaxyl-M·Fludioxonil+amino acids demonstrated a control efficacy of 93.65% against root rot in tobacco floating seedlings. In comparison, the application of Metalaxyl-M·Fludioxonil alone exhibited a control efficacy of 88.42%. The most effective treatment for promoting root growth in tobacco seedlings was Metalaxyl-M·Fludioxonil+amino acids, which resulted in a 54.65% increase in root growth compared to the blank control. This was followed by the application of Metalaxyl-M·Fludioxonil alone, which yielded a 39.53% increase in root growth relative to the blank control. [Conclusions] The application of Metalaxyl-M·Fludioxonil+amino acids is recommended for use in the seedling pools of tobacco seedling cultivation. Specifically, it is advised to incorporate 1 mL of Metalaxyl-M·Fludioxonil and 2 mL of amino acids into each seedling tray prior to their introduction into the seedling pool. This treatment has demonstrated significant efficacy in preventing and controlling root rot in tobacco floating seedlings, while also promoting the health of the root system and ensuring the quality of transplanted tobacco seedlings.
关键词: Tobacco seedlings, Seedbed, Disinfection, Fungicide
Isolation and Identification of Rhizosphere Microorganisms and Endophytes in Pogostemon cablin
摘要: [Objectives] To systematically investigate the microbial community composition of rhizosphere soil and endophytes associated with Pogostemon cablin, and to explore the relationships between endophytes and rhizosphere microorganisms as well as their potential applications. [Methods] Microbial isolates were obtained from rhizosphere soil, root tissues, and stem tissues using the serial dilution and spread plate method. These isolates were identified through morphological characterization, physiological and biochemical assays, and molecular biological techniques. [Results] A total of 18 microbial strains were isolated, including 7 bacterial and 11 fungal strains. Among the bacterial isolates, Pseudomonas spp. and Bacillus spp. were predominant, while the fungal isolates were mainly represented by Aspergillus spp. Certain bacterial strains, notably Pseudomonas spp., exhibited potential abilities for indole-3-acetic acid (IAA) production, nitrogen fixation, and antagonistic activity against pathogenic microorganisms, suggesting their potential utility as biocontrol agents and promoters of plant growth. [Conclusions] This study establishes a foundational understanding of the microbial community characteristics in the rhizosphere and tissues of P. cablin, as well as their roles in plant growth and development.
关键词: Pogostemon cablin, Rhizosphere microorganism, Endophyte, Separation and identification
Composition Analysis of Lycophytes and Ferns in Yunzhongshan Nature Reserve, Fujian Province
摘要: [Objectives] To analyze the composition of lycophytes and ferns in Yunzhongshan Nature Reserve, Fujian Province. [Methods] This study examined the composition of lycophytes and ferns in Yunzhongshan Nature Reserve through field investigations and specimen identification, supplemented by a review of relevant literature. [Results] A total of 84 species of lycophytes and ferns were identified, encompassing 55 genera and 29 families. This included 14 species of lycophytes distributed across 8 genera and 3 families, as well as 70 species of ferns belonging to 47 genera and 26 families. Polypodiaceae and Selaginella were the dominant family and genus, respectively, while oligospecific families and monotypic genera were significant components of lycophytes and ferns in the region. The distribution types of these families were primarily cosmopolitan and tropical. The Germline differentiation degree (SD) was 3.34, and the proportion of rare or endangered species among lycophytes and ferns was 8.33%. [Conclusions] Yunzhongshan Nature Reserve possesses a diverse array of lycophyte and fern species. This study offers valuable scientific insights that can inform efforts related to the conservation of biodiversity, resource utilization and development, species introduction, and system evolution of lycophytes and ferns within the reserve.
关键词: Nature reserve, Lycophytes, Ferns, Composition