| 摘要 |
[Objectives] To explore the neuroprotective mechanism of Qingre Huayu Decoction on rats with acute cerebral ischemia injury. [Methods] SD rats were divided into sham operation group, ischemia model group, low, medium and high dose groups of Qingre Huayu Decoction, with 10 rats in each group. Referring to the MCAO operation model, both the sham operation group and the model group were given normal saline by gavage, and the Qingre Huayu Decoction group was given different doses of Qingre Huayu Decoction by gavage. After the operation, the rats were scored for neurological deficit, neurons were stained with HE, apoptotic cells were detected with TUNEL, and the levels of autophagy and apoptotic proteins in the Ca2+/CaMKKβ-AMPK-mTOR pathway in brain tissue were detected with Western-blot. [Results] Compared with the model group, the neurological function score of Qingre Huayu Decoction Group decreased significantly (P<0.05), the pathological damage of neurons in Qingre Huayu Decoction Group decreased, the proportion of apoptosis-positive cells detected by TUNEL decreased (P<0.05), and the expression of CaMKKβ and AMPK increased, expression of mTOR decreased, expression of Beclin-1 and LC3 increased, and expression of Caspase-3 decreased in Qingre Huayu Decoction Group (P<0.05). [Conclusions] Qingre Huayu Decoction may play a neuroprotective role by activating Ca2+/CaMKKβ-AMPK-mTOR pathway and regulating the level of apoptosis and autophagy. |
