| 摘要 |
[Objectives] To explore the effects of chlorogenic acid from honeysuckle on the secretion enzymes, lipoxygenase A4 (LXA4), and blood biochemical indicators in mice with aluminum induced Alzheimer’s disease (AD). [Methods] Chlorogenic acid was extracted from honeysuckle by ultrasound assisted alcohol extraction method. Seventy mice were randomly divided into normal group, model group, and low, medium and high dose groups of honeysuckle chlorogenic acid. All the mice in each group except for the normal group were given maltol aluminum by intraperitoneal injection to establish models of aluminum induced AD, continuously injected for 5 d and stopped for 2 d, totally poisoned for 8 weeks. Starting from the 5th week of poisoning, the low, medium and high dose groups of honeysuckle chlorogenic acid were given honeysuckle chlorogenic acid solution 40, 80 and 160 mg/kg by gavage, respectively, while the normal group and the model group were fed with an equal volume of distilled water, all once daily, continuously gavaged until the end of the 8th week. At the end of the experiment, the learning memory ability of the mice was tested by Y-type water maze, and the number of tests required to reach the learning standard, the number of memory errors in 20 tests and the error rate of the mice were recorded. The brains of mice were taken to determine the contents of β-secretase, α-secretase, γ-secretase, LXA4 and acetylcholinesterase (AchE) in the homogenates of brain tissues by ELISA, and their blood was taken to determine the biochemical indexes. [Results] Compared with the normal group, the number of learning tests, number of memory errors, error rate and the contents of β-secretase, γ-secretase and AchE in brain tissue of the mice in the model group were all significantly increased (all P<0.05), the contents of LXA4 in brain tissue were significantly decreased (all P<0.05), and the contents of α-secretase did not change significantly (all P>0.05); compared with the model group, the number of learning tests, the number of memory errors, the error rate and the content of β-secretase, γ-secretase and AchE in brain tissue were all significantly reduced (all P<0.05), the content of LXA4 in brain tissue of the high dose group of honeysuckle chlorogenic acid was significantly increased (P<0.05), and there was no significant change in the content of α-secretase in brain tissue of all groups of honeysuckle chlorogenic acid (all P>0.05). Compared with the normal group, the levels of blood glucose, TC, TG, ALT, BUN, Cr and UA in the model group and the levels of TC, TG and BUN in the low- and medium-dose groups of honeysuckle chlorogenic acid were significantly increased (all P<0.05), and the level of HDL-C in the model group and the levels of UA in the medium-and high-dose groups of honeysuckle chlorogenic acid were significantly decreased (all P<0.05); compared with the model group, the levels of blood glucose, ALT, BUN, UA in each group of honeysuckle chlorogenic acid, the levels of TC and Cr in medium and high dose groups of honeysuckle chlorogenic acid, and the level of TG in the high dose group of honeysuckle chlorogenic acid were all significantly lower (allP<0.05), while the level of HDL-C in the medium and high dose groups of honeysuckle chlorogenic acid and the level of total protein in the high dose group of honeysuckle chlorogenic acid were all significantly higher (all P<0.05). [Conclusions] Chlorogenic acid from honeysuckle may improve AD induced by aluminum exposure via regulating related secretory enzymes, LXA4, and various biochemical indicators. |
