| 摘要 |
[Objectives] To establish the HPLC-DAD fingerprint of Xueshuan Xinmaining Capsule (XXC). [Methods] The chromatographic conditions for the analysis of XXC solution were as follows: XSelect HSS T3 column; acetonitrile-0.1% phosphoric acid water was used as mobile phase, gradient elution; flow rate: 1.0 mL/min; column temperature 30 ℃; The injection volume is 10 μL. The quality of XXC samples produced by different manufacturers was evaluated by similarity evaluation and cluster analysis. [Results] In the HPLC-dad fingerprints of 15 batches of XXC, 23 common peaks were identified and 9 peaks were identified, and the similarity was greater than 0.95. According to the results of cluster analysis, 15 batches of XXC samples could be divided into two categories, S2, S5, S6, S7 and S8 batches belonged to category I, and the rest batches belonged to category II. [Conclusions] In this study, a representative and universal identification method of XXC HPLC-DAD fingerprint was established. The method has high precision, stability and repeatability, is simple and reliable, and provides a powerful reference for further improving the quality evaluation system of XXC. |
