| 刊名 | Medicinal Plant |
| 作者 | Jingrong LU， Jiangcun WEI， Xiumei MA， Bing QING， Meiyan QIU， Wen ZHONG |
| 作者单位 | Guangxi International Zhuang Medicine Hospital |
| DOI | 10.19601/j.cnki.issn2152-3924.2024.03.008 |
| 年份 | 2024 |
| 刊期 | 3 |
| 页码 | 35-38 |
| 关键词 | Chuanlianzhu, Thin-layer chromatography (TLC), Extraction process, Rubiadin-1-methyl ether, Content determination |
| 摘要 | [Objectives] To establish a thin-layer chromatography (TLC) method for the determination of rubiadin-1-methyl ether in Yao Medicine Chuanlianzhu (Damnacanthus giganteus). [Methods] A silica gel G thin-layer plate was adopted for TLC. Petroleum ether (60-90 ℃)-chloroform-methanol-water (7：15：3：1) was used as the developing solvent and inspected under ultraviolet lamp (365 nm). The content was determined by Inertsil ODS-3 C18 column (4.60 mm×250 mm, 5 μm), mobile phase: acetonitrile-0.2% phosphoric acid gradient elution, detection wavelength 277 nm, flow rate 1.0 mL/min, column temperature 30 ℃, injection volume 10 μL. [Results] The spots of 10 Chuanlianzhu samples from different origins showed the same color at the same position as the control, and the spots were clear and specific. The injection volume of rubiadin-1-methyl ether showed a good linear relationship in the range of 2.90-145 μg (R=0.999 6). The average recovery rate of rubiadin-1-methyl ether in the low, medium and high dose groups of Yao Medicine Chuanlianzhu was 98.72%, and RSD=1.78%. [Conclusions] This method can effectively identify Yao Medicine Chuanlianzhu medicinal materials and accurately determine the content of rubiadin-1-methyl ether in the medicinal materials. It provides a scientific basis for the development and utilization of Yao Medicine Chuanlianzhu medicinal resources. |
