| 刊名 | Medicinal Plant |
| 作者 | Luyao XIE，Xiaoxia LI，Jing FU，Jinhua LIU，Yaobin HUANG，Xuri WEI，Tingting MO，Shaomian PAN，Lin HUANG，Jiabao MA |
| 作者单位 | Graduate School of Guangxi University of Chinese Medicine； The First Affiliated Hospital of Guangxi University of Chinese Medicine |
| DOI | 10.19601/j.cnki.issn2152-3924.2024.03.001 |
| 年份 | 2024 |
| 刊期 | 3 |
| 页码 | 1-3,21 |
| 关键词 | Aconiti Lateralis Radix Praeparata, Trichosanthis Fructus, Benzoylaconitine compound, Content determination |
| 摘要 | [Objectives] This study was conducted to determine the contents of benzoylmesaconine, benzoylaconitine and benzoylhypacoitine in the decoctions of Heishun pieces, Trichosanthis Fructus and their combination. [Methods] Heishun pieces, Trichosanthis Fructus and their combination were extracted for different time periods, and then grouped. HPLC was performed using an Agilent ZORBAX SB-C18 chromatographic column (4.6 mm×250 mm, 5 μm) and acetonitrile-0.02 mol/L sodium dihydrogen phosphate as the mobile phase at a flow rate of 1 mL/min and a column temperature of 30 ℃, and the sample volume was 20 μL. The detection wavelength was 230 nm. [Results] The total amounts of benzoylmesaconine, benzoylaconitine and benzoylhypacoitine in the single decoction group of Heishun pieces were all significantly different from those in the combined decoction group at corresponding time. [Conclusions] The total content of the benzoylaconitine type increased significantly after the combined decoction of Heishun pieces and Fructus Trichosanthis, which proves the scientificity of "eighteen incompatible medicaments, 19 counteraction" in traditional Chinese medicine to some extent. |
