| 刊名 | Medicinal Plant |
| 作者 | Kaifei LIU， Yingyuan QIN， Linhao LIU， Ye CHU， Huanjin HUANG， Jingwei LUO， Xiaolong CHEN， Yang YANG， Yifei CHEN |
| 作者单位 | College of Pharmacy,Guilin Medical University； Guangxi Key Laboratory of Drug Discovery and Optimization,Guangxi Engineering Research Center for Pharmaceutical Molecular Screening and Druggability Evaluation,College of Pharmacy,Guilin Medical University； Department of Nephrology,Guilin Municipal Hospital of Traditional Chinese Medicine |
| DOI | 10.19601/j.cnki.issn2152-3924.2024.02.007 |
| 年份 | 2024 |
| 刊期 | 2 |
| 页码 | 26-29 |
| 关键词 | Pereskia aculeata Mill.,Ferroptosis,MAPK signal pathway,Colon cancer |
| 摘要 | [Objectives]The paper was to investigate the mechanism by which Pereskia aculeata Mill.regulates ferroptosis and interferes with colon cancer using network pharmacology.[Methods]The effect of P. aculeata on HCT116 cells was observed by examining cell morphology.The intersection of P. aculeata,colon cancer,and ferroptosis targets was determined using the Venny 2.1.0 online platform.DAVID database was used to perform GO and KEGG enrichment analyses. Cytoscape_v3.10.0 was used for network mapping and PPI analysis.[Results]The observation of cell morphology indicated that P. aculeata suppressed the growth of HCT116 cells.GO and KEGG analysis identified 67 genes involved in potential pathways,including the MAPK signaling pathway and Apoptosis,18 of which were located on the MAPK pathway.After conducting PPI visualization analysis and ranking based on Degree values,we identified TP53,TNF,EGFR,MAPK14,and AKT1 as the top five targets with the highest Degree values.[Conclusions]P. aculeata may activate the p53 gene through the MAPK signaling pathway,inducing ferroptosis and ultimately resulting in tumor cell death. |
