| 刊名 | Medicinal Plant |
| 作者 | Jiangcun WEI， Xiumei MA， Meiyan QIU， Bing QING， Jingrong LU， Hong LEI， Xiaodong HUANG， Wen ZHONG |
| 作者单位 | Guangxi International Zhuang Medical Hospital； Guangxi University of Chinese Medicine |
| DOI | 10.19601/j.cnki.issn2152-3924.2024.02.004 |
| 年份 | 2024 |
| 刊期 | 2 |
| 页码 | 15-17，20 |
| 关键词 | Pileostegia tomentellal,TLC identification,Extraction technology,Umbelliferone,Content determination |
| 摘要 | [Objectives]To establish a TLC and content determination method of Pileostegia tomentellal,with umbelliferone as the indicator component.[Methods]TLC identification was performed by silica gel G thin layer plate with n-hexane-ethyl acetate (4：3) as the developing agent,and the plate was examined by UV lamp (365 nm).The umbelliferone content was determined by HPLC:Inertsil ODS-3 C18 column (4.60 mm×250 mm,5 μm); mobile phase acetonitrile-0.2% phosphoric acid gradient elution; detection wavelength 320 nm,flow rate 1.0 mL/min,column temperature 30 ℃,injection volume 10 μL.[Results]The chromatogram of P. tomentellal showed the same color spot in the same position as that of reference medicinal material,and the spot was clear with good specificity.Umbelliferone showed a good linear relationship when the injection volume was 2.63-131.27 μg/mL (R2=0.999 7).The average recovery of umbelliferone in the low,middle and high adding groups of P. tomentellal was 99.57% and the RSD was 2.15%.[Conclusions]The method can effectively identify Yao medicine P. tomentellal and accurately determine the content of umbelliferone in medicinal materials,which will provide a scientific basis for the development and utilization of medicinal resources of Yao medicine P. tomentellal. |
