| 摘要 |
[Objectives] This study was conducted to establish a method for the determination of chlorogenic acid, geniposide, total flavonoids and total triterpenes in Wulan-13. [Methods] The contents of chlorogenic acid and geniposide were determined by HPLC, and the contents of total flavonoids and total triterpenes were determined by an ultraviolet spectrophotometer. [Results] There was a good linear relation between the mass of chlorogenic acid reference substance and the peak area in the range of 0.05-0.45 µg, and the regression equation was Y=2 524.1X+3.194 3, (r=0.999 8). A good linear relationship was found between the mass of gardenoside reference substance and the peak area in the range of 0.776-6.984 µg, and the regression equation was Y=1 670.5X+64.804, (r=0.999 8). There was also a good linear relation between the mass of rutin reference substance and its absorbance in the range of 0.008 08-0.048 48 mg, and the regression equation was Y=12.916X+0.014, (r=0.999). The mass of oleanolic acid reference substance had a good linear relation with its absorbance in the range of 0.004 18-0.020 9 mg, and the regression equation was Y=51.89X-0.083 9, (r=0.999 1). [Conclusions] The content determination method is simple, reliable and reproducible, and suitable for controlling the contents of chlorogenic acid, geniposide, total flavonoids and total triterpenes in Wulan-13. |
