| 摘要 |
[Objectives]This study was conducted to screen lavandulyl flavonoids with anti-inflammatory activity from Sophora flavescens.[Methods]35 compounds were screened from traditional Chinese medicine S. flavescens using the nitric oxide (NO) anti-inflammatory activity model.[Results]Five components,8 (xanthohumol),13 (kurarinol),27 (4-methoxysalicylic acid),28 (b-resorcic acid) and 30 (b-resorcic acid),exhibited significant anti-inflammatory activity,with IC50 values of 5.99,4.76,6.96,3.41 and 5.22 μM,respectively.Especially,8 (xanthohumol) and 13 (kurarinol) were typical lavandulyl flavonoids in S. flavescens,which were worth further exploration.Furthermore,UPLC-Q-Exactive and GNPS molecular networking technique were used for rapid analysis of lavandulyl flavonoids from S. flavescens. A total of 15 components were identified.[Conclusions]This work lays a theoretical foundation for further separation and analysis of lavandulyl flavonoids with anti-inflammatory activity from S. flavescens. |
