| 摘要 |
[Objectives] To investigate the mechanisms and pharmacologic effects of Citri Reticulatae Pericarpium against keloids by network pharmacology systematically. [Methods] TCMSP, Uniprot and BATMAN-TCM databases were used to obtain the active constituents and targets of Citri Reticulatae Pericarpium. "Keloid" was used as key word to search for related therapeutic targets from Drug Bank, OMIM, TTD, and GEO databases. The Chinese medicine compound-target network was constructed by Cytoscape software. Besides, gene ontology (GO) and Kyoto Encyclopedia of genes and genome enrichment analysis were also performed. Afterward, Discovery Studio software was used to assess the interaction of key components and genes. [Results] Five active components of Citri Reticulatae Pericarpium, 773 compound targets and 676 keloid treatment targets were obtained in the databases. After the intersection, there are 47 targets of Citri Reticulatae Pericarpium for treating keloids. Hub genes were identified such as MMP9, IL6, TNF, TP53, and VEGFA, which were enriched in tumor necrosis factor-α, nuclear factor kappa-B, and other signaling pathways. The molecular docking stimulation confirmed the interaction between the MMP9 and three components of Citri Reticulatae Pericarpium. [Conclusions] Citri Reticulatae Pericarpium may play an important role in treating keloids through modulating genes and signaling pathways. The present study sheds light on the mechanisms of active compounds of Citri Reticulatae Pericarpium for the treatment of keloids. |
