| 刊名 | Medicinal Plant |
| 作者 | Yi LI， Qianjiong HUANG， Jinhui JIANG， Guoyang LIN， Chenchen HUANG， Jie GAO |
| 作者单位 | Affiliated Hospital of Youjiang Medical University for Nationalities； Department of Pathology and Pathophysiology, Youjiang Medical University for Nationalities |
| DOI | 10.19601/j.cnki.issn2152-3924.2023.06.015 |
| 年份 | 2023 |
| 刊期 | 6 |
| 页码 | 60-63 |
| 关键词 | Daphnetin, Mastitis, Staphylococcus aureus, Red cell distribution width (RDW), Neutrophil to lymphocyte ratio (NLR), Ratio of platelets to lymph-ocytes (PLR), Systemie Immune Inflammati-on Index (SII) |
| 摘要 | [Objectives] To observe the effects of daphnetin on mastitis induced by Staphylococcus aureus in mice. [Methods] 18 postpartum ICR female mice were used to establish mastitis animal model, and were randomly divided into three groups (A, B, and C) with 6 mice in each group. Group A: blank control group; group B: S. Aureus model group; group C: S. Aureus model+daphnetin group. The experimental groups were injected 1 mL of 1.0×104 CFU/100 μL of S. aureus of along the nipple catheter. The suspension was placed in the 3rd and 4th pairs of mammary glands, and the control group was injected with the same dose of normal saline. On the second day after infection, the rats in group A, B and C were given drugs by gavage, while the rats in group A and B were given normal saline and the rats in group C were given daphnetin once a day for 6 consecutive days. Blood samples were collected from living eyeballs, and blood cells were analyzed by automatic flow cytometer after anticoagulation. [Results] The NLR and Systemie Immune Inflammati-on Index (SII) in the blood of mastitis mice induced by S. aureus were significantly higher than those in the control group (P<0.01), suggesting that neutrophil to lymphocyte ratio (NLR) and SII can be used as diagnostic indicators of mastitis, and the levels of NLR and SII decreased significantly after daphnetin intervention. [Conclusions] NLR and SII showed high levels in mastitis mice, which are valuable for the diagnosis of mastitis and the evaluation of its prognosis. After the intervention of daphnetin, both of them decreased significantly, indicating that daphnetin has a good prognosis trend in mastitis mice induced by S. aureus. |
