| 摘要 |
[Objective] To study the effects of JAG-1 on silencing TRAIP (tumor necrosis factor receptor associated factor interaction protein) after regulating Notch signaling pathway on the proliferation and migration of gastric adenocarcinoma cells. [Methods] Gastric adenocarcinoma cells were categorized into si-NC+DMSO (control+DMSO), si-TRAIP#1+DMSO (transfected with TRAIP+DMSO), si-NC+JAG-1 (control+JAG-1), and si-TRAIP#1+JAG-1 (transfected with TRAIP+JAG-1), and the proliferation of the cells was detected by CCK-8 assay and plate colony formation assay. Transwell assay was used to detect cell migration, and Western blot was adopted to detect the expression of proliferation-associated protein CyclinD1, migration-associated protein MMP2, and key proteins of Notch signaling pathway Notch1, Hes1 and Jagged1. [Results] Compared with siTRAIP#1+DMSO, the gastric adenocarcinoma cells in si-TRAIP#1+JAG-1 group showed increased proliferation and migration (P<0.05), and there was a significant increase in the expression of CyclinD1, MMP2, Notch1, Hes1, and Jagged1 (P<0.05). [Conclusion] After TRAIP knockdown, JAG-1 increased not only the proliferation and migration ability of gastric adenocarcinoma cells, but also the expression of key proteins of Notch signaling pathway Notch1, Hes1, and Jagged1. |
