| 摘要 |
［Objectives］To explore the protective effect of Alpinia zerumbet (Pers.) Burttet Smith ethanol extraction (YSJ) on gastric ulcers. [Methods] The ethanol-induced gastric ulcer model was generated by male Kunming mice. The mice were divided into six groups, including control, model (GU), positive omeprazole enteric-coated capsules (18 mg/kg), YSJ low (1.17 g/kg), medium (2.34 g/kg) and high (4.68 g/kg) dose groups. To observe the state of gastric tissues, hematoxylin and eosin (H&E) staining was applied and immunohistochemistry (IHC) was used to evaluate the expression of nuclear factor kappa-B p65 (NF-κB p65), interleukin 6 (IL-6), tumor necrosis factor-α (TNF-α), and Cyclooxygenase-2 (COX-2) in gastric tissues. The ulcer index, biochemical parameters, and inflammatory proteins were evaluated. In vitro, GES-1 cells were induced by anhydrous ethanol to found gastric ulcer model. The groups were the same as in vivo experiment. Cytotoxicity was tested by MTT and IL-1β, TNF-α, and IL-6 were detected by Elisa assays. [Results] The injuries of gastric tissue in the model group were unambiguously observed and improved after YSJ treatment. The levels of interleukin 1 beta (IL-1β), interleukin 6 (IL-6), interleukin 8 (IL-8), and TNF-α in serum and tissues were decreased (P<0.05 or 0.01), whereas interleukin 4 (IL-4) and prostaglandin E2 (PGE2) were increased after treatment with YSJ (P<0.05 or 0.01). The levels of IL-1β, IL-6, TNF-α, and IL-8 were decreased in YSJ groups, while IL-4 and PGE2 presented the opposite trend. The protein expression of p-NF-κB, NF-κB, p-IκBα,and TNF-α was inhibited after treatment with YSJ (P<0.05 or 0.01). [Conclusions] These results demonstrate that YSJ alleviates the occurrence and development of gastric ulcers via inhibiting inflammation. |
