| 摘要 |
［Objectives］ To observe the effects of Cigu Xiaozhi Formula on miR-378a-3p expression and Hh signaling pathway in TGF-β1 induced and activated LX2 cells. [Methods] Cells were divided into control group, induction group, drug-containing serum group, miR-378a-3p inhibitor group, and miR inhibitor NC group. CCK-8 method was used to detect the cell viability of each group, and flow cytometry was used to detect the apoptosis rate of each group. RT-qPCR was used to detect the expression of miR-378a-3p in each group’s cells, and RT-qPCR and Western blot were used to detect mRNA and protein expression of Shh, Gli1, Gli2, Col-I, and α-SMA in each group’s cells. [Results] Compared with the control group, the cell viability and expression of Shh, Gli1, Gli2, Col-I, and α-SMA mRNA and protein in induction group increased (P<0.01), while the expression of miR-378a-3p decreased (P<0.01). Compared with the induction group, the cell viability and expression of Shh, Gli1, Gli2, Col-I, α-SMA mRNA and α-SMA and Gli2 protein decreased in drug-containing serum group (P<0.05), while cell apoptosis rate and miR-378a-3p expression increased (P<0.01). In miR-378a-3p inhibitor group, cell viability and the expression of Shh, Gli1, Gli2, Col-I, α-SMA mRNA and Gli1, Gli2, α-SMA protein increased (P<0.05, P<0.01), while the apoptosis rate and miR-378a-3p expression decreased (P<0.05, P<0.01). [Conclusions] Cigu Xiaozhi Formula containing serum can upregulate miR-378a-3p expression and downregulate the expression of Gli2 and α-SMA in TGF-β1 induced LX2 cells, thereby inhibiting the activation of LX2 cells and exerting the effects of anti liver fibrosis. |
