| 刊名 | Medicinal Plant |
| 作者 | Xian PENG， Zhenying FU， Jiangcun WEI， Yinghong HUANG， Xianyi SHI， Wei TIAN， Wen ZHONG， Guodong HUANG |
| 作者单位 | Guangxi International Zhuang Medicine Hospital |
| DOI | 10.19601/j.cnki.issn2152-3924.2023.05.005 |
| 年份 | 2023 |
| 刊期 | 5 |
| 页码 | 25-28 |
| 关键词 | Zhuang Medicine Xiaoyan Zhiyang Lotion, Thin layer chromatography (TLC), High-performance liquid chromatography (HPLC), Quality control |
| 摘要 | ［Objectives］To establish the TLC identification methods of Llicis Rotundae Cortex and Mahoniae Caulis and the determination method of syringin content in Zhuang Medicine Xiaoyan Zhiyang Lotion. [Methods] Thin layer chromatography (TLC) was used to qualitatively identify Llicis Rotundae Cortex and Mahoniae Caulis in Zhuang Medicine Xiaoyan Zhiyang Lotion. The quantitative analysis of syringin in Zhuang Medicine Xiaoyan Zhiyang Lotion was carried out by high-performance liquid chromatography (HPLC). Chromatographic conditions are as follows: Luna 5 μm C18 (2) 100 Å (250 mm×4.6 mm, 5 μm) column was adopted; acetonitrile (10%) -water (90%) was as the mobile phase, and the flow rate was 1.0 mL/min; the detection wavelength was 265 nm; the sample size was 5 μL; the column temperature was 30 ℃. [Results] Syringin had a good linear relationship with its peak area in the range of 0.190-2.856 μg (R2=0.999 5). The RSD values of precision, stability and repeatability experiments were all lower than 2.5%, and the average sampling recovery rate (n=6) was 100.37% (RSD=0.88%). [Conclusions] The method had the advantages of simple operation, strong specificity, good repeatability and stability, and can be used for the quality control of Zhuang Medicine Xiaoyan Zhiyang Lotion. |
