| 摘要 |
［Objectives］ To regulate the quality of Wanjinxiang Shushuang Ointment through simultaneously quantifying menthol, camphor, 1, 8-cineole, linalool, borneol and caryophyllene oxide by QAMS (quantitative analysis of multi-components by single-marker). [Methods] The method was performed using an Agilent DB-WAX (30 m×0.32 mm, 0.25 μm) polyethylene glycol chromatographic column; with nitrogen employed as a carrier gas. The constant pressure was 4.73 psi; and the injection temperature was 240 ℃, with a shunt ratio：of 10：1; The hydrogen flame ion detector with a detector temperature of 240 ℃; the injection volume was 0.3 μL. To verify the accuracy and applicability of QAMS, the results were compared with those obtained using the internal standard method (naphthalene). [Results] In Wanjinxiang Shushuang Ointment, menthol, camphor, 1, 8-cineole, linalool, borneol, caryophyllene oxide and naphthalene were well separated by the same chromatography with good linearity in their respective ranges (R≥0.999 2). The average recoveries were 99.66%, 101.03%, 98.07%, 98.24%, 101.39%, and 103.39% with RSDs of 0.69%, 1.52%, 1.25%, 1.94%, 1.44%, and 2.74%, respectively. The QAMS is similar to the internal standard method. [Conclusions] This simple, accurate method with high precision, separation and reproducibility can serve as a reference for the quality control of Wanjinxiang Shushuang Ointment. |
