| 摘要 |
[Objectives] To investigate whether Huaier (Trametes robiniophila Murr) can reverse oxaliplatin resistance of colorectal cancer and its molecular mechanism. [Methods] CCK8 assays were used to analyze the effect of Huaier on the survival rate of HCT8 and HCT8/L cells, Flow cytometry was used to verify the apoptosis rate and flow cytometry detected the cell cycle, gene expression lever of P-glycoprotein was detected by RT-qPCR assays, and the protein expression lever was detected by western blot. [Results] Huaier can significantly reduce the cell viability of HCT8 and HCT8/L cells, induce cell apoptosis and arrest the cell cycle at G1/G0 phase. Huaier could inhibit the expression lever of MDR1 mRNA and inhibit the protein expression of P-glycoprotein, P85α, p-AKT, BCL-2, CDK4, CyclinD1, and promote the expression of BAX, Cleaved caspase-3 and cleaved caspase-9 in drug-resistant colorectal cancer cells. Through compared Huaier group with the 740Y-P group and the co-treated group, it shows that Huaier can reverse oxaliplatin resistance in colorectal cancer through PI3K/AKT signaling pathway. [Conclusions] This study shows that Huaier can reverse oxaliplatin resistance of colorectal cancer by inducing apoptosis and arresting cell cycle through PI3K/AKT signaling pathway. |
