Analysis of Chemical Components of Cocculus laurifolius DC. Based on Liquid Chromatography-Mass Spectrometry
刊名 Medicinal Plant
作者 Lintong HAN, Yuzhe LI, Chengxin LAN, Bolin BAO, Yajie WEI, Haicheng WEN, Wei WEI, Xiaoman ZHONG
作者单位 Guangxi University of Chinese Medicine
DOI 10.19601/j.cnki.issn2152-3924.2025.01.003
年份 2025
刊期 1
页码 16-18
关键词 Cocculus laurifolius DC., UPLC-Q-Exactive HRMS, Chemical component, Alkaloids, Flavonoids
摘要 [Objectives] To analyze the main chemical components in Cocculus laurifolius DC. by ultra-high performance liquid chromatography-quaternary rod/electrostatic field orbital hydrazine high resolution mass spectrometry. [Methods] Using Welch AQ-C18 chromatographic column (150 mm×2.1 mm, 1.8 μm), gradient elution was performed with 0.1% formic acid aqueous solution (A)-methanol (B) as the mobile phase, and electrospray ESI ionization source and simultaneous mass spectrometry scanning mode of positive and negative ions were used. [Results] 26 kinds of chemical component were identified or inferred, including 3 organic acids, 5 flavonoids, 4 alkaloids, 1 coumarin and 13 others. [Conclusions] The UPLC-Q-Exactive HRMS technique is simple, which lays a foundation for the drug-efficacy material basis and medicinal quality evaluation of C. laurifolius DC.