摘要 |
[Objectives] The paper was to elucidate the specific hyperparasitic fungal species that are associated with coffee leaf rust. [Methods] Tissue isolation, sample humidification, and three-point inoculation techniques were employed to isolate, culture, and purify the hyperparasitic fungi responsible for coffee leaf rust. The purified strains were identified using traditional morphological techniques and molecular biology methods. [Results] Four strains were isolated, specifically BS21(Cladosporium cladosporioides), BS34 (C. tenuissimum), BS62 (C. cladosporioides), and BS75 (C. colombiae). [Conclusions] The findings of this research will contribute novel insights into the biological control of coffee leaf rust. |