| 刊名 | Plant Diseases and Pests |
| 作者 | Mingrui LIN， Zhicheng YU， Jinshui LIN， Kunxiu CAI， Zhaoyang HUANG， Xuejiao HE |
| 作者单位 | Fujian Institute of Tropical Crops |
| DOI | 10.19579/j.cnki.plant-d.p.2024.04.008 |
| 年份 | 2024 |
| 刊期 | 4 |
| 页码 | 37-39 |
| 关键词 | Curcuma alismatifolia; Explants; Cluster buds induction; Proliferation |
| 摘要 | ［Objectives］ The paper was to study the tissue culture nursery technology of Curcuma alismatifolia ‘Kimono Rose’. ［Methods］ By sterilizing and inoculating explants derived from disparate regions of C. alismatifolia, we identified the most optimal explants and optimized the culture conditions for cluster buds induction and proliferation. This was achieved by incorporating MS medium with varying concentrations of 6-BA and NAA, thereby establishing a foundation for the large-scale production of C. alismatifolia tissue culture seedlings. ［Results］ The optimal explant for C. alismatifolia was identified as a lateral bud. The most effective cluster buds induction medium was determined to be MS+6-BA 5 mg/L+NAA 0.1 mg/L+sucrose 25 g/L+agar 6.5 g/L. The optimal cluster buds proliferation medium was found to be MS+6-BA 3 mg/L+NAA 0.1 mg/L+sucrose 25 g/L+agar 6.5 g/L. ［Conclusions］ The findings of this study can provide a foundation for the enhancement of the industrialized breeding system of tissue culture propagation of C. alismatifolia. |
