| 摘要 |
［Objectives］ This study was conducted to clarify the biological characteristics of the pathogen Phyllosticta capitalensis, the causal agent of freckle disease on Cavendish banana in Hainan Province, China. ［Methods］ The impact of various nutritional and environmental factors, including media, carbon sources, nitrogen sources, temperature, pH and light on the growth and sporulation of P. capitalensis was assessed using two distinct methods: mycelium growth rate and blood counting chamber. ［Results］ The mycelial growth and sporulation of P. capitalensis on different media exhibited notable differences. The use of banana leaf extract dextrose agar (BLEAD) and carrot agar (CA) was observed to facilitate rapid mycelial growth. The potato dextrose agar (PDA) and potato sucrose agar (PSA) were conducive to the production of conidia. The utilization of distinct carbon and nitrogen sources exerted a pronounced influence on the growth of P. capitalensis. Maltose, dextrose, fructose, and casein acid hydrolysate were the preferred substrates for mycelial growth. The tested carbon and nitrogen sources did not significantly stimulate conidial production, whereas dextrose and NaNO3 were found to favor sporulation. The optimal temperature for mycelial growth and conidial production was determined to be 28 and 32 ℃, respectively. No mycelial growth was observed at 5 ℃. Active mycelial growth was observed at pH 6-10, with pH 6-7 being particularly conducive to sporulation. Complete darkness was conducive to mycelial growth and sporulation. ［Conclusions］ It is recommended that BLEDA and PDA should be incubated at 28 ℃ for 14 d in the dark for the purpose of mycelial growth and sporulation of P. capitalensis, respectively. |
