| 摘要 |
[Objectives] The paper was to establish a TLC identification method for Ensete wilsonii. [Methods] Using β-sitosterol as the reference, the effects of preparation methods of test solutions, developing solvents, developing distances and color developing agents on TLC analysis were investigated, and the best TLC conditions for E. wilsonii were determined. [Results] The test solution prepared with 90% methanol solvent was dotted on TLC silica gel G plate, and developed with dichloromethane-toluene-methanol=10：5：1.5 as the developing solvent. Then the plate was sprayed with 10% sulfuric acid ethanol solution, and dried with hot blast for color development. Finally, the plate was examined under an ultraviolet lamp at 365 nm. The TLC results of E. wilsonii obtained showed good separation and color development effect, and the spots were clear and characteristic. [Conclusions] This method is safe, specific, and easy to operate, and can be used as a TLC identification method for E. wilsonii. |
