| 摘要 |
[Objectives] The paper was to develop a detection system applicable to major quarantine diseases affecting citrus in Zhaoqing. [Methods] qPCR was utilized for the detection of citrus Huanglongbing (HLB) and citrus bacterial canker disease (CBCD). Additionally, RT-PCR was employed for both single and dual RT-PCR assay of citrus HLB, citrus tristeza virus (CTV), and citrus yellow vein clearing virus (CYVCV). The dual RT-PCR assay system was optimized regarding reagent concentration and annealing temperature to establish a robust dual RT-PCR assay system suitable for the identification of citrus HLB, CTV and CYVCV in Zhaoqing. [Results] The established qPCR techniques for the detection of CBCD and HLB exhibited a high degree of specificity. The specific fragment sizes associated with the established single or dual RT-PCR assay techniques were as follows: HLB at 1 160 bp, CTV at 273 bp, and CYVCV at 614 bp, respectively. The amplification products generated by these techniques were characterized by their clarity, specificity, and sensitivity. [Conclusions] The established method for the detection of citrus diseases is systematic and capable of addressing the diverse needs of various users. |
