| 刊名 | Animal Husbandry and Feed Science |
| 作者 | Liu Yueyue1#, Zhang Yu1#, Zhu Yuwei1, Guo Xiaozhen1, Yang Shifa1, Yin Bin1, Zhao Zengcheng1, Huang Zhongli1, Wu Jiaqiang2,3*, Lin Shuqian1* |
| 作者单位 | 1. Institute of Poultry Science, Shandong Academy of Agricultural Sciences, Shandong Engineering Research Center for Animal Health Products, Jinan 250100, China; 2. Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan 250100, China; 3. Shandong Provincial Collaborative Innovation Center of Cell Biology, Shandong Normal University, Jinan 250014, China |
| DOI | 10.19578/j.cnki.ahfs.2024.01-03.003 |
| 年份 | 2024 |
| 刊期 | 1 |
| 页码 | 13-20 |
| 关键词 | Porcine astrovirus; Epidemic situation; Genotype analysis; Isolation and identification |
| 摘要 | [Objective] The paper was to identify, isolate, and characterize porcine astrovirus in Shandong Province between 2021 and 2023. [Method] A total of 1 025 samples of porcine diarrhea samples were collected from various regions of Shandong Province between January 2021 and October 2023. The samples were tested by RT-PCR, followed by sequencing and phylogenetic analyses of the polymerase. [Result] The total positive rate of PAstV was 34.6% (355/1025). The respective proportions of individuals infected with PAstV 1, PAstV 2, PAstV 4 and PAstV 5 were 25.4% (90/355), 28.2% (100/355), 35.2% (125/355) and 22.5% (80/355), respectively. Additionally, mixed infection was observed. Meanwhile, 849 samples of healthy pigs were tested by RT-PCR, and the results demonstrated that the total positive rate of PAstV was 8.13% (69/849). Of these, the proportion of PAstV 1, PAstV 2 and PAstV 4 infection was 27.5% (19/69), 37.7% (26/69) and 40.6% (28/69) ,and a mixed infection also existed. Further sequencing and characterization of some the selected isolates revealed low sequence identities (56.2%) with known PAstV strains, indicating the presence of novel types or genotypes of PAstVs. Furthermore, the isolation conditions of porcine astrovirus were optimized, resulting in the purification of a pure PAstV-4 strain (designated PAstV-4-GRF1). The virus was found to exhibit typical astroviral morphology, with nucleotide identity ranging from 89.9 to 95.4% with previously published PAstV-4 strains. Then, macrovirus transcriptome sequencing showed that 88.30% of the GRF1 samples were mammalian astroviruses. By species classification, PAstV 4 and PAstV 2 accounted for 21.79% and 0.32%, respectively. Phylogenetic tree analysis showed that the c15050 fragment was identical to the GRF-1 sequencing fragment of the isolated strain, and exhibited the highest homology with the Hunan PAstV 4 sequence MK460231 in China. [Conclusion] As the inaugural isolated PAstV-4 strain, it furnishes pivotal material for the investigation of the biological and pathogenic properties of this virus as well as for the prospective development of relevant biological and diagnostic reagents. |
