| 刊名 | Animal Husbandry and Feed Science |
| 作者 | Zhou Huishuang1,2, Lin Shuqian 2,3, Li Guiming2, Liu Yueyue2,3, Yang Shifa2,3, Zhao Zengcheng2,3, Huang Zhongli2.3, Lian Ruirui1,2, Wang Yunchao4, Wan Renzhong1*, Yin Bin2,3* |
| 作者单位 | 1.Veterinary Pharmacology and Toxicology, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian 271018, China; 2. Poultry Institute, Shandong Academy of Agricultural Sciences, Jinan 250023, China; 3. Shandong Provincial Engineering Center for Animal Health Products, Jinan 250023, China; 4. Blue Horizon Eco-agriculture, Dongying 257091, China |
| DOI | 10.19578/j.cnki.ahfs.2024.01-03.001 |
| 年份 | 2024 |
| 刊期 | 1 |
| 页码 | 1-8 |
| 关键词 | Tea polyphenols; H9C2; Network pharmacology; Thermal damage |
| 摘要 | [Objective] The paper was to investigate the action targets and pathways of tea polyphenols in alleviating heat stress–induced injury by using network pharmacological analysis and an H9C2 cell model. [Method] First, the corresponding targets of tea polyphenols were obtained from the PubChem database. Then, the core targets were screened based on topological parameters. The relevant metabolism pathways of tea polyphenols related to diseases were identified through GO functional annotation and KEGG signaling pathway enrichment. Moreover, common targets for thermal injury and targets of tea polyphenols were obtained. Then, GO functional annotation was performed to explore the pathway of tea polyphenols in alleviating heat stress damage. H9C2 cells were cultured at 42 °C to construct the heat stress model, and the cells were treated with 10 µg/mL tea polyphenols. The key genes were confirmed using RT-PCR technology. [Result] The study yielded 364 targets corresponding to tea polyphenols, including 68 core targets. These targets are related to various biological processes such as involve oxidative stress, cancer, lipopolysaccharide-mediated signaling pathways, antiviral responses, regulation of cellular response to heat, apoptosis, and cellular lipid metabolic metabolism. Tea polyphenols alleviate thermal damage by targeting BCL2, HSP90AA1, HSPA1A, JUN, MAPK1, NFKB1, NFKBIA, NOS3, and TP53. Moreover, 10 mg/L tea polyphenols were found to upregulate the transcription levels of Hsp70, HO-1, NQ-O1, Nrf2, and MAPK1, and the transcription levels of Bax/Bcl2, p38, and JNK were downregulated to alleviate the heat stress–induced injury. [Conclusion] Tea polyphenols may enhance the antioxidant ability of H9C2 cells and inhibit cell apoptosis, thereby reducing heat stress injury. |
