| 刊名 | Animal Husbandry and Feed Science |
| 作者 | Zhao Daoquan, Xie Guoqiang, Wu Huihui, Yu Xiao, Li Yingnan, Li Xianming |
| 作者单位 | Henan Academy of Fishery Sciences; Henan Yellow River Basin Yiluo River Aquatic Biological Field Scientific Observation and Research Station |
| DOI | 10.19578/j.cnki.ahfs.2022.04-06.008 |
| 年份 | 2022 |
| 刊期 | 2 |
| 页码 | 41-44 |
| 关键词 | SCSA; Sperm preservation solution; DNA fragmentation index; Sperm motility |
| 摘要 | The quality of fish sperm is an important factor affecting artificial reproduction.Since the sexual maturation of male and female is not synchronized or for the fish that need to kill the male fish for sperm,sperm preservation solution is used to preserve the sperm temporarily during reproduction,in order to meet the needs of large-scale reproduction.Therefore,the preservation effect of sperm preservation solution directly affects the artificial reproduction.The quality of sperm in the preservation solution can be judged macroscopically by microscopic examination,but it is not accurate,while sperm chromatin structure analysis(SCSA)can quantitatively judge the quality of sperm in the preservation solution,thus providing guarantee for reproduction.The Zhaos sperm preservation solution of Pseudobagrus hwanghoensis has been verified by artificial breeding and SCSA analysis for many times,and it is better than Hanks sperm preservation solution.When the DNA fragmentation index is less than or equal to 0.15,the sperm is vigorous and the effect is obvious. |
