| 摘要 |
[Objective] The paper was to understand the physiological, enzymatic and molecular changes of Acipenser schrencki induced by heat exposure. [Method] The effects of heat exposure on A. schrencki were investigated. The temperature was increased from(18± 0.5) ℃ by 0.08 ℃/min and respectively kept at(32±0.5) ℃ for 2, 4 and 6 h. The respiration rate, related biochemical indicators and heat shock protein 70(Hsp 70) mR-NA expression were investigated. [Result] At(32±0.5) ℃, the activities of total protein, albumin, globulin, urea nitrogen, ceatinine, malondialde-hyde, aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase fluctuated dramatically from 2 h to 6 h. Meanwhile, the respi-ration rate and Hsp 70 m RNA expression in heart, liver and spleen were significantly increased from 2 h to 6 h. In comparison, the lower respira-tion rate and tiny fluctuations in activities of biochemical indicators were consistent with the lower Hsp 70 expression. [Conclusion] During the process of heat stress, A. schrencki seems to be sensitive to such temperature, and manifests generally consistent gill breathing, biochemical indica-tors and Hsp70 gene expression, implying better heat tolerance. |
