| 摘要 |
[Objectives] This study was conducted to investigate the effects of adding compound probiotics on the growth performance and intestinal flora of Kunming mice. [Methods] Twelve healthy 2-week-old Kunming male mice with body weight of (11.09 ± 0.43) g were selected. They were randomly divided into two treatment groups, namely blank control group (NC) and compound probiotics group (CB + LR + BS), with six mice in each group. The two groups were fed with commercial basal diet, and the compound probiotic experimental group was fed with basal diet supplemented with compound probiotics, in which the contents of Clostridium butyricum spores, Lactobacillus reuteri and Bacillus subtilis spores were 1 × 1010, 1 × 1011 and 1 × 1010 CUF/kg, respectively. The body weight, feed intake and water intake of mice were counted every 4 d, and the experimental period was 14 d. On the 14th day, the cecal contents of the mice were collected for analysis. [Results] There was no significant change in body weight and feed intake when compound probiotics were added to the diet. However, the addition of compound probiotics reduced the abundance of harmful bacteria such as Escherichia coli, urease-negative Helicobacter typhlonius and Salmonella enterica, while increasing the abundance of beneficial bacteria such as Anaerostipes hadrus, and the contents of IgG and IgM increased significantly (P < 0.05). [Conclusions] In summary, the addition of compound probiotics could significantly improve the structure of intestinal microbial flora, increase the quantity of beneficial bacteria, reduce the quantity of harmful bacteria, and improve the immune function of mice. |
