| 刊名 | Agricultural Biotechnology |
| 作者 | Ruiling LYU1, Zhenxing XIE1, Qiwen TAO1, Liuyao WANG1, Peng WU1, Shiming LI1,2, Jun YANG3, Yongliang ZHENG1 |
| 作者单位 | 1. Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountain, Huanggang Normal University, Huanggang 438000, China; 2. Department of Food Science, Rutgers University, New Brunswick, NJ 07102, USA 3. Huanggang Academy of Agricultural Sciences, Huanggang 438000, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2024.04.010 |
| 年份 | 2024 |
| 刊期 | 4 |
| 页码 | 40-45 |
| 关键词 | Morchella septimelata; Polysaccharides extraction; Response surface design |
| 摘要 | Morchella septimelata polysaccharide (MSP) possesses multiple biological activities. In this study, double enzyme method was used to extract polysaccharides from Morchella septimelata. The basis of single factor experiment and response surface design was used to optimize the extraction process of polysaccharides. The optimal extraction conditions of polysaccharide were as follows: extraction time 2 h, extraction temperature 40.5 ℃, solid-liquid ratio 1:15 (w/v), and the amount of double enzyme (cellulase: β-glucanase, mass ratio 1:2) 2.0 %. Under these conditions, the extraction yield of polysaccharide reached (14.86±0.12)%. |
