| 刊名 | Agricultural Biotechnology |
| 作者 | Le WANG1, Jiao CHEN2， Jiali WENG2, Jinxing FU2, Wenqin SU2, Bingmiao GAO2, Qin CHEN3 |
| 作者单位 | 1. Department of Food and Pharmacy, Hainan Health Management College, Chengmai 571999, China; 2. School of Pharmacy, Hainan Medical University, Haikou 571199, China; 3. Hainan Open University, Haikou 570208, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2024.04.008 |
| 年份 | 2024 |
| 刊期 | 4 |
| 页码 | 31-37 |
| 关键词 | Stichopus japonicus; Proteomics; Protein extraction; Caenorhabditis elegans; Anti-aging |
| 摘要 | The sea cucumber Stichopus japonicus is one of the "Eight Treasures of Seafood" and contains a number of bioactive components involved in multiple physiological and pharmacological functions. Proteins and peptides are generally considered to be responsible for these beneficial properties. In this study, a total of 3 478 proteins and 17 390 peptides were identified in Stichopus japonicus by proteomics methods. Among them, 4 proteins were involved in 8 metabolic pathways, especially oxidative phosphorylation and cell senescence. Subsequently, the lifespan assay and oxidative stress test were performed to investigate the peptides prepared from sea cucumber protein hydrolyzate using the aging model of Caenorhabditis elegans. The results of the anti-aging experiment demonstrated that high-dose peptides significantly prolonged the lifespan of nematodes (30.50%), and improved their capacity to inhibit oxidative stress. The results provide evidence supporting the development of bioactive proteins and peptides derived from Stichopus japonicus as functional foods and lay the foundation for the research of an anti-aging drug. |
