| 摘要 |
[Objectives] This study was conducted to investigate the effects of piperidine derivatives on the proliferation and apoptosis of tumor cells (Hele). [Methods] The target end product (piperidine derivative) was synthesized through a series of organic reactions. The MTT assay was adopted to detect the effect of piperidine derivative on the proliferation activity of Hele cells. The ROS fluorescence probe method was used to detect the changes of reactive oxygen species. The JC-1 method was applied to detect the changes of MMP in Hele cells. Flow cytometry was adopted to detect the apoptosis of Hele cells. [Results] The cell survival rates were 70.84%, 65.46% and 54.48% when the drug concentration was 100, 110 and 120 μmol/L, respectively. When the drug concentration increased to 120 μmol/L, the cell survival rate decreased by nearly half. The fluorescence intensity of active oxygen in the control group was 1, and when the drug concentrations were 100, 110 and 120 μmol/L, the fluorescence intensity of active oxygen was, respectively, 1.315, 1.478 and 1.677, which were higher than that in the control group. The red/green fluorescence intensity of the MMP control group was 1.819, and that of drug groups was, respectively, 1.643, 1.164 and 0.665, which were lower than that of the control group. The apoptosis rates were 10.79%, 22.91% and 38.54% at the drug concentrations of 100, 110 and 120 μmol/L, respectively, showing a concentration dependent effect. The results showed that the piperidine derivative could inhibit the proliferation of Hele cells and induce apoptosis, which was positively correlated with the concentration. [Conclusions] This study provides theoretical basis and reference for the anti-tumor research of piperidine. |
