| 刊名 | Agricultural Biotechnology |
| 作者 | Jian ZHANG1, Qi QI1, Yonghou LIANG1, Zhimin DONG1, Gui WANG1, Xueyuan ZHOU2* |
| 作者单位 | 1. Hetao College, Bayannur 015000, China; 2. Inner Mongolia Autonomous Region Maternal and Child Health Hospital, Hohhot 010021, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2022.01.007 |
| 年份 | 2022 |
| 刊期 | 1 |
| 页码 | 60-66 |
| 关键词 | Bovine spermatozoon; Annexin V; Immunomagnetic beads; Frozen sexed semen |
| 摘要 | [Objectives] This study was conducted to develop a molecular marker immunomagnetic bead sorting technology method that can specifically identify dead spermatozoa. [Methods] This study first confirmed the specific binding of Annexin V to dead bovine spermatozoa, and tried to remove dead spermatozoa in semen combining with the immunomagnetic bead technology, so as to improve the separation efficiency of target spermatozoa in the process of sex-controlled semen preparation on a flow cytometer. [Results] The spermatozoon motility, membrane integrity and mitochondrial activity after sorting and the rate of dead spermatozoa during the on-machine X/Y separation were all improved in different degrees (P<0.05), indicating that the technical process design could effectively remove some dead spermatozoa, and there was no significant effect on frozen sexed semen prepared from the separated X or Y spermatozoa (P>0.05), indicating that the technical process did not cause additional damage to the spermatozoa. [Conclusions] Combining the specificity of Annexin V with the immunomagnetic bead method could effectively remove dead spermatozoa from bovine spermatozoa, and significantly reduce the rate of dead spermatozoa in bovine permatozoa during sex-controlled separation (P<0.05). The method developed can effectively improve the production efficiency of frozen sexed semen of dairy cows, reduce the production cost, and promote the industrial application of the product. |
