| 刊名 | Agricultural Biotechnology |
| 作者 | Guixia YANG, Jie LIU, XiujuaN WANG, Fenglan ZHANG, Kun XIN, Chunli KONG |
| 作者单位 | Jiaxiang Hengcheng Inspection Detection Co., Ltd., Jining |
| DOI | DOI:10.19759/j.cnki.2164-4993.2024.02.003 |
| 年份 | 2024 |
| 刊期 | 2 |
| 页码 | 8-9,19 |
| 关键词 | Benzo [a] pyrene; High performance liquid chromatography; Fluorescence detector |
| 摘要 | In this study, an optimized high performance liquid chromatography-fluorescence detector (HPLC-FL) method for the determination of benzo[a]pyrene in edible oil was established. HPLC was performed with Thermo Fisher Scientific C18 column (250 mm×4.6 mm, 5 μm) as the chromatographic column and acetonitrile and water as the mobile phase, and the excitation wavelength and emission wavelength of fluorescence detector were 286 and 430 nm, respectively. The response was high, and the linear range was 0.5-10.0 ng/ml. The lowest limit of detection was 0.11 ng/ml, and the average recovery was 92.5%. This method is suitable for quantitative analysis of benzo[a]pyrene content in edible oil. |
