Response Surface Optimization of Ultrasound-assisted Aqueous Two-phase Extraction of Sweet Potato Leaf Polysaccharides
刊名 Agricultural Biotechnology
作者 Yuxuan WU, Junqing WANG, Wenjie WANG
作者单位 College of Bioengineering,Qilu University of Technology (Shandong Academy of Sciences),Jinan Baotuquan Liquor-making Co.
DOI DOI:10.19759/j.cnki.2164-4993.2023.03.024
年份 2023
刊期 3
页码 106-112
关键词 Aqueous two-phase system; Ultrasound assistance; Response surface optimization; Sweet potato leaf polysaccharides
摘要 [Objectives]The ultrasound-assisted aqueous two-phase extraction of sweet potato leaf polysaccharides was studied.[Methods]With the yield of sweet potato leaf polysaccharides as the index,the aqueous two-phase extraction system was determined,and the optimal extraction conditions were optimized by single-factor experiments and response surface methodology.[Results]The optimal parameters were ethanol concentration 25.68%,liquid-to-material ratio 55.83,and ultrasonic treatment time 38.33 min.Under these conditions,the yield of sweet potato leaf polysaccharides could reach 20.646 mg/g.[Conclusions]The ethanol/ammonium sulfate aqueous system is a rapid and efficient method for extracting sweet potato leaf polysaccharides,which is of great significance for the application of sweet potato leaf extract as a natural food additive.