Analysis of Differential Metabolites in Selenium-enriched Rice Based on Extensive Non-targeted Metabolome
刊名 Agricultural Biotechnology
作者 Biaojin ZHANG ,Xiaoyu CHANG, Tianhua TU, Xiangxi ZHANG, Tingcan DAI
作者单位 Institute of Quality, Safety and Standards of Agricultural Products Research, Jiangxi Academy of Agricultural Sciences
DOI DOI:10.19759/j.cnki.2164-4993.2023.03.023
年份 2023
刊期 3
页码 101-105,112
关键词 Rice; Selenium; Non-targeted metabolome; Differential metabolites
摘要 [Objectives]Selenium(Se)-enriched rice is the main type of Se-enriched agricultural product developed in China,and this study aimed to understand the impact of selenium application on the metabolites in rice.[Methods]Se-enriched rice was prepared by foliar application of a sodium selenite aqueous solution,and high-throughput analysis of differential metabolites in Se-enriched rice was conducted based on extensive non-targeted metabolome.[Results]There were significant differences in metabolites between Se-enriched rice and ordinary rice,and a total of 535 differential metabolites were identified.Among them,420 metabolites in Se-enriched rice were upregulated,accounting for 78.5%,far higher than downregulated metabolites.The enrichment differences of three KEGG metabolic pathways,including cysteine and methionine metabolism,zeatin biosynthesis,and arachidonic acid metabolism,reached a significant level,indicating that selenium enrichment had a significant regulatory effect on the metabolism of sulfur-containing amino acids,the synthesis of natural cytokinin zeatin,and arachidonic acid bioactive components in rice.[Conclusions]The results can provide a theoretical basis for the production of Se-enriched rice.