| 刊名 | Agricultural Biotechnology |
| 作者 | Xiangyu ZHANG，Kai ZHANG， Bin WEN， Dingsheng YUAN， Chao YANG， Yuying LI ，Liangde KUANG， Rui YANG， Congyan LI， Jie ZHENG ，Yongjun REN， Zhiqiang GUO ，Li TANG， Yang JI， Min LEI， Dengping HUANG， Xiaohong XIE， Cuixia ZHANG |
| 作者单位 | Sichuan Animal Science Academy/Key Laboratory of animal genetics and breeding in Sichuan Province |
| DOI | DOI:10.19759/j.cnki.2164-4993.2023.03.015 |
| 年份 | 2023 |
| 刊期 | 3 |
| 页码 | 66-69 |
| 关键词 | Meat rabbit; Muscle; High-throughput sequencing; Nucleotide bias |
| 摘要 | [Objectives]miRNAs play an important role in the proliferation and differentiation of different myoblasts.This study was conducted to elucidate the complex genetic mechanisms that affect the meat production performance of Sichuan white rabbits and reveal the regulatory role of miRNAs in their muscle growth and meat quality formation.[Methods]Three constructed skeletal muscle libraries of Sichuan white rabbits aged six months were sequenced by the solexa technology to identify known miRNAs,predict new miRNAs and construct an expression profile of muscle miRNAs.[Results]A total of 511 known miRNAs and 42 miRNAs were detected in 34089472 pure sequences,and the proportion of miRNAs with a length of 22 nt was the highest.The number of known miRNA sequences accounted for 71.38%of pure sequences,which was much higher than the proportion of other types of RNAs.The proportion of sequences from exons was 0.38%,indicating a low degree of mRNA degradation in the samples.Base U had the highest proportion at the first position,and the bases with the highest proportions at positions 8 and 10 were U and A,respectively.Muscle-specific miRNAs(miR-1,miR-133,and miR-206)ranked in the top 10 in terms of expression level.The number and expression levels of new miRNAs were lower than those of known miRNAs.The length distribution,base bias at different positions and expression profile characteristics of miRNAs might be related to the biological function of miRNAs in regulating muscle proliferation and differentiation and the action mechanisms with target genes.[Conclusions]The identification and expression of miRNAs in muscle tissues of Sichuan white rabbits will help to understand the complex molecular mechanisms of meat production performance and provide a theoretical basis for the functional research of miRNAs in meat rabbits. |
