| 刊名 | Agricultural Biotechnology |
| 作者 | Xu CHANG， Yanhui WANG ，Ximing LIU ，Yunge MA ，Yunhao LIU ，Xiaobo CHEN， Mengyao SUN， Liyan LI |
| 作者单位 | 1. Medical School, Huanghe Science and Technology College, Zhengzhou 450063, China; 2. College of pharmacy, Henan University, Kaifeng 450046, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2023.04.024 |
| 年份 | 2023 |
| 刊期 | 4 |
| 页码 | 110-114,118 |
| 关键词 | Fructus Aurantss Immaturus; Total flavonoids; Optimization of extraction conditions; UVB radiation resistance |
| 摘要 | [Objectives] Optimum extraction conditions of total flavonoids from Fructus Aurantii Immaturus (TFFAI) and its resistance activity to ultraviolet radiation were investigated in present research. [Methods] The optimal extraction conditions of TFFAI were determined by single factor and orthogonal experiments, and the survival rate of TFFAI on HaCaT cells irradiated with UVB rays was investigated.It's antioxidant capacity was determined by ABTS. [Results] The results showed that the highest yield of TFFAI was obtained with 70% ethanol at a solid-to-liquid ratio of 1:50 (w/v) and 40 ℃ for 1.5 h by single-factor and orthogonal experiments. Total flavonoids (0.25-1.0 mg/ml) could significantly improve the survival rate of HaCaT cell line. Meanwhile, the maximum absorption peak of TFFAI was found at 283 nm, and in-vitro antioxidant experiment identified that TFFAI had a good clearance rate to ABTS.It suggestes that TFFAI could protect the cells from UVB damage by absorption of UVB rays and anti-oxidation. [Conclusion]TFFAI played a protective role on UVB irradiated cells through UVB ultraviolet absorption and antioxidant pathways. |
