| 刊名 | Agricultural Biotechnology |
| 作者 | Shuai LI ，Xinhui WANG ，Yota TATARA ，Shigeki HAMADA ，Takuya KOZEKI， Kaoru KOJIMA ，Takashi YOSHIDA |
| 作者单位 | Department of Biochemistry and Molecular Biology Department of Stress Response Science Faculty of Agriculture |
| DOI | DOI:10.19759/j.cnki.2164-4993.2023.04.022 |
| 年份 | 2023 |
| 刊期 | 4 |
| 页码 | 99-104 |
| 关键词 | Glycosaminoglycan; Hyaluronan; Hyaluronate lyase; Paenibacillus yunnanensis; Paennarthrobacter nicotinovorans |
| 摘要 | Hyaluronate lyases were obtained from two types of naturally isolated bacterial strains Paenibacillus yunnanensis and Paennarthrobacter nicotinovorans.PyHL(form P.yunnanensis)in the culture supernatant of the bacteria was purified by two steps of column chromatography.The enzyme showed the molecular mass of 74 kDa by SDS-PAGE and the maximal activity at pH 5.0,35℃.PyHL maximally degraded hyaluronate by an endo-type manner,and showed low degradation activity toward chondroitin sulfates.Dermatan sulfate was not the substrate.PnHL(from P.nicotinovorans)in the culture supernatant of the bacteria was purified by two steps of column chromatography.The enzyme showed the molecular mass of 70 kDa by SDS-PAGE and the maximal activity at pH 6.0,30℃.Genomic analysis of P.nicotinovorans on the bases of the internal amino acid sequences of PnHL. |
