| 刊名 | Agricultural Biotechnology |
| 作者 | Tao LIU;Wenlai REN;Yuqin LIANG;Yubo WANG;Wei LIU;Xing WANG;Yanpo CAO |
| 作者单位 | 1. School of Landscape and Ecological Engineering, Hebei University of Engineering, Handan 056000, China; 2. Institute of Cash Crops, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China; 3. Wen-an County Agriculture and Rural Bureau, Langfang 065800, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2024.01.002 |
| 年份 | 2024 |
| 刊期 | 1 |
| 页码 | 5-8 |
| 关键词 | Asparagus; Anthocyanins; Gene mapping; Purple |
| 摘要 | [Objectives] This study was conducted to find out regulatory genes related to purple in spears of asparagus (Asparagus officinalis L.). [Methods] The stable asparagus inbred line JX1513-5 (the base of the spear is purple) and JLV1718-7(the base of the spear is green) were used as parents to study the genetic law of purple/green traits in their offspring. [Results] The results showed that the purple in the basal part of asparagus spear was controlled by a pair of alleles, and purple was dominant over green. The F2 segregation population was resequenced by the bulk segregation analysis (BSA) method, and the purple trait in the basal part of asparagus spear was located in the interval of 24.51-25.08 Mb on Chr07 chromosome, which included 47 genes. According to the annotation information, three candidate genes were screened out: LOC109849403, LOC109849430 and LOC109849442. The candidate genes were verified by real-time fluorescence quantitative PCR (qRT-PCR), and finally LOC109849442 was obtained as the candidate gene for controlling the purple/green trait in the basal part of asparagus spear. [Conclusions] This study lays a foundation for the breeding of new asparagus varieties and molecular marker-assisted breeding. |
