| 刊名 | Agricultural Biotechnology |
| 作者 | Jiaqi DING, Ziang YI, Qi QIU, Chenzhong JIN, Taoli LIU* |
| 作者单位 | College of Agriculture and Biotechnology, Hunan University of Humanities, Science and Technology, Loudi 417000, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2025.03.001 |
| 年份 | 2025 |
| 刊期 | 3 |
| 页码 | 1-5 |
| 关键词 | Rice; OsKMP; Tissue expression pattern; Bioinformatics analysis |
| 摘要 | Kinesins are a superfamily of proteins widely present in eukaryotes, playing crucial roles in plant cell wall assembly, cell elongation regulation, gravity sensing, and fertility control. In this study, bioinformatics analysis of the OsKMP2 gene (LOC_Os02g28850) was performed using online tools such as ExPASy-ProtParam, ProtScale, CD-search, and DNAMAN software. Additionally, qRT-PCR was employed to analyze the tissue expression pattern of OsKMP2. The results showed that the molecular weight of the OsKMP2 is 118.397 28 kDa, and it is a hydrophilic and unstable acidic protein. Secondary structure prediction revealed that it primarily consists of α-helices (69.45%), random coils (25.19%), and extended strands (5.36%). The gene was expressed in various rice tissues, with the highest expression level observed in leaves. These results indicate that the OsKMP2 gene exhibits high evolutionary conservation and functional diversity in rice. |
