Effects of Different Fractions of Baeckea frutescens L. on Proliferation of Human Colon Cancer LOVO Cells in Vitro
刊名 Agricultural Biotechnology
作者 Xiaofan WANG1#, Keyi ZHOU1#, Hong XUE1, Jialong LU1, Feiyang WEI1,Pinhong LI1, Caijian PAN1, Zhiqiang CHEN2*, Haijiao ZHANG3, Changfu HU3, Xin JIANG2*, Dengfeng ZOU1*
作者单位 1. Guilin Medical University, Guilin 541199, China; 2. Affiliated Hospital of Guilin Medical University, Guilin 541001, China; 3. Nanxishan Hospital of Guangxi Zhuang Autonomous Region, Guilin 541004, China
DOI DOI:10.19759/j.cnki.2164-4993.2025.02.018
年份 2025
刊期 2
页码 89-93
关键词 Extract of Baeckea frutescens L.; Human colon cancer LOVO cell; Inhibition; Effect
摘要 [Objectives] This study was conducted to investigate the inhibitory effects of different extraction fractions of Baeckea frutescens L. on the proliferation of human colon cancer LOVO cells in vitro. [Methods] Leaves were separated from the aerial part of B. frutescens, immersed in distilled water and treated by ultrasound for half an hour. The volatile oil in B. frutescens leaves was extracted by steam distillation. The leaves after removing the volatile oil were extracted by reflux condensation, and then the extract was concentrated into fluid extract. The organic solvent extraction method was applied to extract the fluid extract until the organic phase was nearly colorless, and the extracts were combined, and concentrated into fluid extract, which was then freeze-dried into powder for later use. A cytotoxicity experiment was carried out on human colon cancer LOVO cells with different extraction fractions of B. frutescens as test drugs. Cell activity was detected by MTT assay. [Results] The petroleum ether fraction and chloroform fraction of B. frutescens had no inhibitory effect on the proliferation of human intestinal cancer LOVO cells in vitro. The ethyl acetate fraction and n-butanol fraction of B. frutescens had inhibitory effects on the proliferation of human colon cancer LOVO cells in vitro in a dose-dependent manner. The IC50 of ethyl acetate was 398.94 μg/ml, and that of n-butanol was 617.02 μg/ml. [Conclusions] The ethyl acetate and n-butanol extraction fractions from B. frutescens have inhibitory effects on the proliferation of human colon cancer LOVO cells in vitro in a dose-dependent manner.