| 刊名 | Agricultural Biotechnology |
| 作者 | Shu WANG1, Xianzhi JIANG2, Juan LUO2, Qing LI2*, Yaohong LIU2, Lijun LI2, Lei YI2, Dayunzi HUANG2 |
| 作者单位 | 1. Anhua County Market Supervision Administration, Yiyang 413500, China; 2. Hunan GRG Metrology & Test Co., Ltd., Changsha 410007, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2025.01.015 |
| 年份 | 2025 |
| 刊期 | 1 |
| 页码 | 64-68 |
| 关键词 | Liquid chromatography-tandem mass spectrometry; Milk tea; Perfluorinated Compound |
| 摘要 | [Objectives] This study was conducted to establish a detection method for the simultaneous determination of 18 perfluorinated compounds (PFCs) in milk tea by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). [Methods] The samples were first subjected to precipitation of proteins by potassium ferrocyanide solution and zinc acetate solution, and then extracted by acetonitrile and detected by LC-MS/MS. Next, sodium chloride was added, and after vortex centrifugation, the acetonitrile layer was dried by blowing with nitrogen. Subsequently, 1 ml of methanol was added to prepare a test solution. An ACQUITY UPLC BEH C18 (2.6 m, 2.1 mm× 100 mm) chromatographic column was used for liquid phase separation, and gradient elution was performed using 10 mmol ammonium acetate solution-acetonitrile as the mobile phase. The MS detection adopted the MRM mode for acquisition, positive and negative ion mode switching for simultaneous determination, and external standard method for quantification. [Results] In the linear ranges of the target substances, the correlation coefficients R2 were all greater than 0.99. The detection limits of the method was in the range of 0.001-0.05 μg/kg, and the quantitation limits were in the range of 0.03-0.20 ng/L. The recovery values ranged from 72.8% to 110.5%. [Conclusions] This method has high sensitivity and good accuracy, and thus strong practical value. |
