| 刊名 | Agricultural Biotechnology |
| 作者 | Xintian HUANG1#, Keyi ZHOU1#, Dengfeng ZOU1, Jinfeng ZHOU1, Haijiao ZHANG2, Xiaofan WANG1, Jingyi JIANG1, Zhiqiang CHEN3*, Changfu HU2*, Xin JIANG3* |
| 作者单位 | 1. Guilin Medical University, Guilin 541199, China; 2. Department of Vascular Surgery, Nanxishan hospital of guangxi zhuang autonomous region,, Guilin 541004, China; 3. The Affiliated Hospital of Guilin Medical University, Guilin 541001, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2024.06.017 |
| 年份 | 2024 |
| 刊期 | 6 |
| 页码 | 78-82,91 |
| 关键词 | Hirudin; HepG 2 cells and Huh-7 cells; Proliferation; Migration; Inhibition |
| 摘要 | [Objectives] This study was conducted to explore the proliferation inhibition of hirudin on hepatocellular carcinoma HepG 2 cells and Huh-7 cells. [Methods] Hirudin solutions of different concentrations (2.0, 2.5, 3.0, 3.5, 4.0 mg/ml) were used to treat HepG 2 cells and Huh-7 cells. The effects of different hirudin concentrations on the proliferative activity of HepG2 and Huh-7 cells were detected by CCK-8 assay, and the IC50 values were calculated. A living/dead cell double staining experiment was conducted to observe the fluorescence of cells under a fluorescent microscope, so as to assess the inhibitory effect of different concentrations of hirudin on the proliferation of HepG2 and Huh-7 cells. A cell scratch assay was carried out, and an inverted microscope was employed to observe the healing of the scratched areas, so as to assess the impact of hirudin on the migratory and invasive capabilities of hepatocellular carcinoma HepG2 and Huh-7 cells. [Results] (i) The results of CCK-8 assay indicated that compared with the blank control group, the proliferation inhibition rates of both hepatocellular carcinoma HepG2 and Huh-7 cells increased with the concentration of hirudin increasing, demonstrating that hirudin had an inhibitory effect on the proliferative activity of these cells. Specifically, the IC50 values for HepG2 and Huh-7 were found to be 3.5 and 4.0 mg/ml. (ii) The living/dead cell double staining experiment revealed that the number of living cells in the hirudin-treated group decreased significantly compared with the control group, while the number of dead cells increased markedly, indicating an inhibitory effect of hirudin on the proliferation of HepG2 and Huh-7 cells. (iii) The results of cell scratch assay showed that the healing degree of the scratched areas in the hirudin-treated groups was significantly lower than that of the control group, indicating a reduction in cell growth and migration capabilities. [Conclusions] Hirudin exhibited a significant inhibitory effect on the proliferation of hepatocellular carcinoma HepG2 and Huh-7 cells. |
