| 刊名 | Agricultural Biotechnology |
| 作者 | Bei LIU1, Ting DING2, Yufeng ZHENG1, Fang WU1, Mi TIAN1, Qian WANG1, Ting HU1* |
| 作者单位 | 1. Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, College of Biology and Agricultural Resources, Huanggang Normal University, Huanggang 438000, China ; 2. Hong’an Public Inspection and Testing Center, Huanggang 438400, China |
| DOI | DOI:10.19759/j.cnki.2164-4993.2024.06.012 |
| 年份 | 2024 |
| 刊期 | 6 |
| 页码 | 46-51 |
| 关键词 | Lactarius deliciosus; Polysaccharide; Ultrasonic-assisted extraction; Response surface analysis; Antioxidant activity |
| 摘要 | [Objectives] Polysaccharide was extracted from Lactarius deliciosus by ultrasonic-assisted method to improve polysaccharide yield. [Methods] Five variables including extraction temperature, extraction time, ultrasonic power, ultrasonic time and material-to-liquid ratio were selected for single factor experiments. The extraction process of L. deliciosus polysaccharide was optimized by response surface analysis, and its antioxidant activity was evaluated by measuring its total reduction ability and DPPH free radical scavenging capacity. [Results] The optimal extraction conditions were determined as follows: material-to-liquid ratio 1:35 g:ml, ultrasonic power 462 W, ultrasonic time 10 min, extraction time 110 min and extraction temperature 90 ℃. Under these conditions, the extraction rate of polysaccharide was 10.83%±0.03%. The antioxidant test results showed that when the mass concentration of L. deliciosus polysaccharide was 0.5 mg/ml, its absorbance and DPPH free radical scavenging rate reached their maximum values, which were 3.274 and 41.27%, respectively. The L. deliciosus polysaccharide had good antioxidant properties. [Conclusions] This study provides a theoretical basis for further development and utilization of L. deliciosus polysaccharide in the future. |
