Development of Quantitative Analysis Method of Carbendazim in Tomatoes by High-Performance Liquid Chromatography with Fluorescence Detection (HPLC-FLD)
刊名 Agricultural Biotechnology
作者 Haijian LIU1, Jiaxin LI1, Minyan CEN1, Ziyu WANG1, Chang LIU1,2*
作者单位 1. College of Biological and Agricultural Resources, Huanggang Normal University, Huanggang 438000, China; 2. Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal University, Huanggang 438000, China
DOI DOI:10.19759/j.cnki.2164-4993.2024.06.009
年份 2024
刊期 6
页码 34-36,41
关键词 Carbendazim; Tomato; High-performance liquid chromatography; Analytical method
摘要 Carbendazim belongs to the benzimidazole fungicides, which can be used for control lots of fungi pathogens. High-performance liquid chromatography is frequently used for the analysis of carbendazim in all kinds of samples. In most occasions, the developed methods were applied for the simultaneous detection of a huge number of pesticides. Thus, an analytical method via UPLC-FLD was developed, and the sample preparation process was optimized by studying the effect of extraction solvent, approach, time and purification absorbent on the recovery rate of carbendazim. The results showed the optimized method for analysis was ultrasonication-assisted extraction with acetonitrile for 1 min, and subsequent purification by C18. In this occasion, the established analytical method of carbendazim in tomato samples displayed good linearity, accuracy and precision.