| 刊名 | Animal Husbandry and Feed Science |
| 作者 | Zhang Yanda1, Pan Huiqing1, Zhang Wentong1, Zhao Qi1, Xu Shuijing1, Yang Qian2*, Wang Xin3* |
| 作者单位 | 1. Fujian Provincial Enterprise Technology Center of Fujian Beidi Pharmaceutical Co., Ltd., Ningde 355300, China; 2. School of Pharmacy, Fujian Medical University, Fuzhou 350108, China; 3. Institute of Environment, Resource, Soil and Fertilizer, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China |
| DOI | 10.19578/j.cnki.ahfs.2025.01-06.006 |
| 年份 | 2025 |
| 刊期 | 1 |
| 页码 | 35-40 |
| 关键词 | Lactobacillus plantarum; Separation and identification; Probiotic performance; Safety |
| 摘要 | [Objective] The study aimed to develop microecological preparations for poultry as potential substitutes for certain antibiotics. [Method] Acid-producing bacteria were isolated and purified from chicken cecal chyme, followed by evaluation of their antibacterial properties and molecular biological identification. Additionally, their in vitro tolerance and safety were experimentally assessed. [Result] A lactic acid bacterium with notable antibacterial properties was isolated and identified as Lactobacillus plantarum. This strain exhibited inhibitory effects against the pathogenic indicator bacteria Escherichia coli and Staphylococcus aureus, with maximum inhibition zone diameters measuring 8.95 and 6.43 mm, respectively. In vitro tolerance tests demonstrated that this strain was capable of growth within a pH range of 4-8, tolerated bile salt concentrations between 0.10% and 0.30%, and adapted to simulated gastric and intestinal fluid environments. Safety test revealed that the strain exhibited no hemolytic activity and was deemed safe in mouse safety assessments. [Conclusion] A chicken-derived L. plantarum strain has been identified, exhibiting notable antibacterial activity, stress tolerance, probiotic efficacy, and safety. This strain holds significant potential and market prospects for the development of microecological preparations. |
